Superresolution Optical Microscopy

Professor Barry R. Masters received a PhD from The Weizmann Institute of Science, Israel, an MS degree from Florida State University, and a BS from The Polytechnic Institute of Brooklyn, New York. Professor Masters was Visiting Scientist in the Department of Biological Engineering at the Massachusetts Institute of Technology, Visiting Scholar in the History of Science Department at Harvard University, Visiting Professor in the Department of Ophthalmology at the University of Bern, and Professor in Anatomy and Cell Biology at the Uniformed Services University of the Health Sciences. He was a Visiting Professor in Japan, India, Australia, Switzerland, Taiwan, Turkey, The United Kingdom, P. R. China, and Egypt. He is a Fellow of the American Association for the Advancement of Science (AAAS), the Optical Society of America (OSA), and the international society for optics and photonics (SPIE). From 1999 to 2000, Professor Masters was an AAAS Congressional Science and Engineering Fellow and served as Legislative Assistant in the United States Congress. He is a recipient of the Vogt Prize for Ophthalmic Research (highest Swiss award for research in ophthalmology) with Dr. Matthias Böhnke in 1999 for their work on “confocal microscopy of the cornea.” Professor Masters has published 87 refereed papers, 143 book chapters and proceedings articles, 105 abstracts, and 239 book reviews. He is the editor or author of 10 books, among them Noninvasive Diagnostic Techniques in Ophthalmology, Confocal Microscopy and Multiphoton Excitation Microscopy: The Genesis of Live Cell Imaging, and Handbook of Biomedical Nonlinear Optical Microscopy (with Peter So). Masters wrote the essay “What Is Light?” (translated into 15 languages) for the International Commission for Optics (ICO), to inaugurate the International Year of Light, 2015. He lectures worldwide on the Responsible Conduct of Research, biomedical ethics, critical thinking, and biomedical photonics. His scholarly interests include developments in the fields of in vivo microscopy of the human eye and skin, biomedical imaging and spectroscopy, fractal analysis of branching vascular patterns, ethics in science and medicine, the history of nineteenth- and twentieth-century physics, science education, and art and science.

About the Author.- Epigraph.- Preface.-  Part I Development of Microscopes.- 1 Connections Between Light, Vision, and Microscopes.- 2 Concepts and Criteria of Resolution.- 3 Aberrations and Artifacts Confound Optical Resolution.- 4 Insights in the Development of Light Microscopes.- 5 Ernst Abbe and His Contributions to Optics.- 6 Abbe's Theory of Image Formation in the Microscope.- 7 Helmholtz's Contributions on the Theoretical Limits to the Resolution of the Microscope.- 8 Further Insights to Abbe's Theory of Image Formation in the Microscope Based on Diffraction.- 9 Mathematical Description of Abbe's Theory of Image Formation in the Microscope Based on Diffraction.- Part II Optical Techniques to Enhance Contrast in the Microscope.- 10 Richard Zsigmondy and Henry Siedentopf's Ultramicroscope.- 11 Light-Sheet Fluorescence Microscopy (LSFM).- 12 Phase Microscopy to Enhance Contrast.- Part III Far-Field Superresolution Optical Microscopy.- 13 Structured Illumination Microscopy (SIM).- 14 Stimulated Emission Depletion (STED) Microscopy and Related Techniques.- 15 Localization Microscopy with Active Control.- 16 Coda: Trade-Offs, Cautions, and Limitations of Far-Field Superresolution Optical Microscopes.- Appendix: Annotated Bibliography of Key Publications Relevant to Abbe's Beiträge zur Theorie des Mikroskops 1873.- Index.

"The book is a must for anyone working in optics as well as for anyone interested in science in general. It is very clearly written, touches on many historical aspects of optics and has many photos supporting the text information." (Daniela Dragoman, Optics & Photonics News, osa-opn.org, July 16, 2020)