Synthetic Biology

Re-Factoring Glycolytic Genes for Targeted Engineering of Catabolism in Gram-Negative Bacteria.- Negative Regulation Gene Circuits for Efflux Pump Control.- Methods for Generating Microbial Co-Cultures that Grow in the Absence of Fixed Carbon or Nitrogen.- Synthetic Biology with an All E. coli TXTL System: Quantitative Characterization of Regulatory Elements and Gene Circuits.- Transformation of an Exotic Yeast Species into a Platform Organism: A Case Study for Engineering Glycolipid Production in the Yeast Starmerella bombicola.- Sortase A-Assisted Metabolic Enzyme Ligation in Escherichia coli for Enhancing Metabolic Flux.- Construction of CRISPR Libraries for Functional Screening.- Targeted Genome Editing Using DNA-Free RNA-Guided Cas9 Ribonucleoprotein for CHO Cell Engineering.- Dynamic Management of Codon Compression for Saturation Mutagenesis.- Designed Transcriptional Regulation in Mammalian Cells Based on TALE- and CRISPR/dCas9.- Combinatorial Evolution of DNA with RECODE.- Genome Editing in Penicillium chrysogenum Using Cas9 Ribonucleoprotein Particles.- Targeted Gene Knockout Using Nuclease-Assisted Vector Integration: Hemi- and Homozygous Deletion of JAG1.- Programming Biomaterial Interactions Using Engineered Living Cells.- Rapid Control of Genome Editing in Human Cells by Chemical-Inducible CRISPR-Cas Systems.- Production of a Human Cell Line with a Plant Chromosome.- Bacterial Genome Editing with CRISPR-Cas9: Taking Clostridium beijerinckii as an Example.- CRISPR-Cas9-Mediated Genome Editing and Transcriptional Control in Yarrowia lipolytica.- Construction and Integration of a Synthetic MicroRNA Cluster for Multiplex RNA Interference in Mammalian Cells.- Bio-Algorithmic Workflows for Standardized Synthetic Biology Constructs.- Engineering Genomes with Genotype Specification Language.- Automated Visualization of Genetic Designs Using DNAplotlib.- DNA-Based Analog Computing.- DNA Assemblywith the DATEL Method.- EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.- Designing and Assembling Plasmids for Construction of Escherichia coli Biosensor for Vibrio cholerae Detection.- A Rapid Combinatorial Approach to Assembling Synthetic Prokaryotic and Eukaryotic Protein Expression Vectors.- Standardized Cloning and Curing of Plasmids.- STAR: A Simple TAL Effector Assembly Reaction Using Isothermal Assembly.