Haemopoiesis -

Haemopoiesis

A Practical Approach

N. G. Testa, G. Molineux (Herausgeber)

Buch | Softcover
320 Seiten
1992
Oxford University Press (Verlag)
978-0-19-963365-4 (ISBN)
119,95 inkl. MwSt
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The techniques used in experimental haematology are relevant to many areas of basic and medical research. This volume provides a detailed guide to many of the most important methods in use in this field, from cell culture to "in vivo" assays.
The cells and regulators involved in haemopoiesis are well characterized, providing biochemists and cell and molecular biologists with an exciting system in which to study normal and abnormal growth and differentiation. Rapid progress has also been made in the application of research techniques to human cells, giving rise to new therapeutic approaches to diseases such as leukaemia. This is a detailed guide to the most important methods in use in this area today, and
will be of major interest to haematologists and researchers in many areas of basic and medical research.

1. In vivo assays for multipotential and marrow repopulating cells ; 2. Enrichment of progenitor cell populations from murine and human haemopoietic tissues ; 3. In vitro clonal assays for murine multipotential and lineage restricted myeloid progenitor cells ; 4. Long-term mouse bone marrow cultures ; 5. Clonal and long-term cultures using human bone marrow ; 6. Isolation and culture of factor-dependent haemopoietic cell lines ; 7. Development of haemopoietic cells in liquid culture ; 8. Analysis of growth factor binding to haemopoietic cells ; 9. Signal transduction ; 10. Retroviral gene transfer into haemopoietic cells ; 11. Assays of the haemopoietic microenvironment ; 12. The visualization of haemopoietic and stromal cell types ; 13. Autologous bone marrow transplantation using cultured marrow cells in patients with leukaemia ; 14. Analysis of gene expression in haemopoietic cells ; 15. Molecular analysis of clonality and transplantation in haemopoiesis

Reihe/Serie Practical Approach Series ; 111
Zusatzinfo halftones, line drawings, tables
Verlagsort Oxford
Sprache englisch
Maße 156 x 234 mm
Gewicht 558 g
Themenwelt Medizinische Fachgebiete Innere Medizin Hämatologie
Medizinische Fachgebiete Innere Medizin Kardiologie / Angiologie
Studium Querschnittsbereiche Infektiologie / Immunologie
Naturwissenschaften Biologie Genetik / Molekularbiologie
Naturwissenschaften Biologie Zellbiologie
ISBN-10 0-19-963365-7 / 0199633657
ISBN-13 978-0-19-963365-4 / 9780199633654
Zustand Neuware
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