SMC Complexes

Using Cell Cycle Restricted Alleles to Study the Chromatin Dynamics and Functions of the Structural Maintenance of Chromosome (SMC) Complexes In Vivo.- Degradation of S. cerevisiae Cohesin with the Auxin-Inducible Degron System.- Efficient Depletion of Fission Yeast Condensin by Combined Transcriptional Repression and Auxin-Induced Degradation.- Conditional Mutation of SMC5 in Mouse Embryonic Fibroblasts.- High-Throughput Allelic Replacement Screening in Bacillus subtilis.- Identifying Functional Domains in Subunits of Structural Maintenance of Chromosome (SMC) Complexes by Transposon Mutagenesis Screen in Yeast.- Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes.- Knocking in Multi-Functional Gene Tags into SMC Complex Subunits Using Gene Editing.- Chromosome Conformation Capture with Deep Sequencing to Study the Roles of the Structural Maintenance of Chromosomes Complex In Vivo.- Analysis of the Chromosomal Localization of Yeast SMC Complexes by Chromatin Immunoprecipitation.- Analysis of Cohesin Association to Newly Replicated DNA through Nascent Strand Binding Assay (NSBA).- Preparation of Cell Cycle Synchronized Saccharomyces cerevisiae Cells for Hi-C.- Dissecting DNA Compaction by the Bacterial Condensin MukB.- In Vivo and In Vitro Assay for Monitoring the Topological Loading of Bacterial Condensins on DNA.- A Protocol for Assaying the ATPase Activity of Recombinant Cohesin Holocomplexes.- In Vitro Detection of Long Noncoding RNA Generated from DNA Double-Strand Breaks.- Tracking Bacterial Chromosome Dynamics with Microfluidics-Based Live Cell Imaging.- Live-Cell Fluorescence Imaging of RecN in Caulobacter crescentus under DNA Damage.- Microinjection Techniques in Fly Embryos to Study the Function and Dynamics of SMC Complexes.- Purification and Biophysical Characterization of the Mre11-Rad50-Nbs1 Complex.- Three-Dimensional Thermodynamic Simulation of Condensin as a DNA-Based Translocase.- Molecular Dynamics Simulations of Condensin-Mediated Mitotic Chromosome Assembly.