The ESCRT Complexes

Analysis of the Archaeal ESCRT Apparatus.- Genetic and Cell Biology Methods to Study ESCRTs in Drosophila melanogaster.- Functional Analysis of ESCRT-Positive Extracellular Vesicles in the Drosophila Wing Imaginal Disc.- Subcellular Localization of ESCRT-II in the Nematode C. elegans by Correlative-Light Electron Microscopy.- Proximity Ligation Assay (PLA) to Determine the Endosomal Localization of ESCRT Subunit in Virus-Infected Cells.- Immuno-Localization of ESCRT Proteins in Virus-Infected Cells by Fluorescence and Electron Microscopy.- Single Cell Fluorescence Ratio Image Analysis for Studying ESCRT Function in Receptor Trafficking.- Genetic and Biochemical Analyses of Yeast ESCRT.- Live Imaging of ESCRT Proteins in Microfluidically-Isolated Hippocampal Axons.- Studying the Spatial Organization of ESCRTs in Cytokinetic Abscission Using the High Resolution Imaging Techniques SIM and Cryo-SXT.- Three Dimensional Surface Rendering of ESCRT Proteins Microscopy Data UsingUCSF Chimera Software.- Transient Expression of ESCRT-Components in Arabidopsis Root Cell Suspension Culture-Derived Protoplasts.- Crystallization and Biophysical Approaches for Studying the Interactions between the Vps4-MIT Domain and ESCRT-III Proteins.- Biochemical Approaches to Studying C. elegans ESCRT Function In Vitro.- Purification of Recombinant ESCRT-III Proteins and Their Use in Atomic Force Microscopy and In Vitro Binding and Phosphorylation Assays.- Assessment of ESCRT Protein CHMP5 Activity on Client Protein Ubiquitination by Immunoprecipitation and Western Blotting.- Purification of Plant ESCRT Proteins for Polyclonal Antibody Production.- Genetic and Cytological Methods to Study ESCRT Cell Cycle Function in Fission Yeast.- ESCRT Mutant Analysis and Imaging of ESCRT Components in the Model Fungus Ustilago maydis.- Genetic Suppressor Screen Using an Inducible FREE1-RNAi Line to Detect ESCRT Genetic Interactors in Arabidopsis thaliana.- Screening of Interactions with the ESCRT Machinery by a Gaussia princeps Split Luciferase-Based Complementation Assay.- RNA Interference-Mediated Inhibition of ESCRT in Mammalian Cells.