Chromatin Immunoprecipitation -

Chromatin Immunoprecipitation

Methods and Protocols
Buch | Softcover
243 Seiten
2018 | Softcover reprint of the original 1st ed. 2018
Humana Press Inc. (Verlag)
978-1-4939-8467-1 (ISBN)
117,69 inkl. MwSt
This up-to-date volume includes protocols that illustrate the broad use of chromatin immunoprecipitation (ChIP) and ChIP-related methods in a variety of biological research areas. The collection also includes protocols designed to improve the performance of ChIP for specific applications.  Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Chromatin Immunoprecipitation: Methods and Protocols features techniques, including bioinformatic analysis of ChIP data, will be of interest to a very broad research community in the fields of biochemistry, molecular biology, microbiology, and biomedicine.

ChIP and ChIP-Related Techniques: Expanding the Fields of Application and Improving ChIP Performance.- Considerations on Experimental Design and Data Analysis of Chromatin Immunoprecipitation Experiments.- How to Combine ChIP with qPCR.- Analysis of Protein-DNA Interaction by Chromatin Immunoprecipitation and DNA Tiling Microarray (ChIP-on-Chip).- Chromatin Immunoprecipitation from Mouse Embryonic Tissue or Adherent Cells in Culture, Followed by Next Generation Sequencing.- Chromatin RNA Immunoprecipitation (ChRIP).- DNA Accessibility by MNase Digestions.- Characterization of the Nucleosome Landscape by Micrococcal Nuclease-Sequencing (MNase-Seq).- ChIP-re-ChIP: Co-Occupancy Analysis by Sequential Chromatin Immunoprecipitation.- Sm-ChIPi: Single-Molecule Chromatin Immunoprecipitation Imaging.- Chromatin Immunoprecipitation of Skeletal Muscle Tissue.- Chromatin Immunoprecipitation Assay in the Hyperthermoacidophilic Crenarchaeon, Sulfolobus acidocaldarius.- Using Intra-ChIP to Measure Protein-DNA Interactions in Intracellular Pathogens.- Native Chromatin Immunoprecipitation-Sequencing (ChIP-Seq) from Low Cell Numbers.- MOBE-ChIP: Probing Cell Type-Specific Binding Through Large-Scale Chromatin Immunoprecipitation.- Multiplexed ChIP-Seq Using Direct Nucleosome Barcoding: A Tool for High-Throughput Chromatin Analysis.- Analysis of ChIP-Seq Data in R/Bioconductor.- Spike-In Normalization of ChIP Data Using DNA-DIG-Antibody Complex.

Erscheint lt. Verlag 14.8.2018
Reihe/Serie Methods in Molecular Biology ; 1689
Zusatzinfo 26 Illustrations, color; 9 Illustrations, black and white; X, 243 p. 35 illus., 26 illus. in color.
Verlagsort Totowa, NJ
Sprache englisch
Maße 178 x 254 mm
Themenwelt Naturwissenschaften Biologie Biochemie
ISBN-10 1-4939-8467-5 / 1493984675
ISBN-13 978-1-4939-8467-1 / 9781493984671
Zustand Neuware
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