Erythropoiesis

An Introduction to Erythropoiesis Approaches.- Using the Zebrafish as an Approach to Examine the Mechanisms of Vertebrate Erythropoiesis.- Mouse Models of Erythropoiesis and Associated Diseases.- Dissecting Regulatory Mechanisms Using Mouse Fetal Liver-Derived Erythroid Cells.- Stress Erythropoiesis Model Systems.- Approaches for Analysis of Erythroid Cell Parameters and Hemoglobinopathies in Mouse Models.- Functional Analysis of Erythroid Progenitors by Colony-Forming Assays.- Analyzing the Formation, Morphology, and Integrity of Erythroblastic Islands.- Flow Cytometry (FCM) Analysis and Fluorescence-Activated Cell Sorting (FACS) of Erythroid Cells.- Analysis of Erythropoiesis Using Imaging Flow Cytometry.- Flow Cytometric Analysis of Erythroblast Enucleation.- High-Resolution Fluorescence Microscope Imaging of Erythroblast Structure.- Chromatin Immunoprecipitation (ChIP) with Erythroid Samples.- Chromosome Conformation Capture (3C and Higher) with Erythroid Samples.- Genome Editing of Erythroid Cell Culture Model Systems.- In Vitro Erythroid Differentiation and Lentiviral Knockdown in Human CD34+ Cells from Umbilical Cord Blood.- Growing and Genetically Manipulating Human Umbilical Cord Blood-Derived Erythroid Progenitor (HUDEP) Cell Lines.- Good Manufacturing Practice (GMP) Translation of Advanced Cellular Therapeutics: Lessons for the Manufacture of Erythrocytes as Medicinal Products.