The Extracellular Matrix

The Complex Interplay between Extracellular Matrix and Cells in Tissues.- Method for Studying ECM Expression: In Situ RT-PCR.- Visualizing the Supramolecular Assembly of Collagen.- Steady-state and Pulse-chase Analyses of Fibrillar Collagen.- Methods for Isolation and Characterization of Sulfated Glycosaminoglycans from Marine Invertebrates.- Analysis of Proteoglycan Synthesis and Secretion in Cell Culture Systems.- Analysis of UDP-Sugars from Cultured Cells and Small Tissue Samples.- Methods for Hyaluronan Molecular Mass Determination by Agarose Gel Electrophoresis.- Hyaluronan Pericellular Matrix: Particle Exclusion Assay.- Determination of Cell Surface Hyaluronan through Flow Cytometry.- Method for Detecting Hyaluronan in Isolated Myenteric Plexus Ganglia of Adult Rat Small Intestine.- Analyzing Hyaluronidases in Biological Fluids.- Method for Studying the Physical Effect of Extracellular Matrix on Voltage-dependent IonChannels Gating.- Methods for Monitoring Matrix-Induced Autophagy.- Method for Determining Gelatinolytic Activity in Tissue: In Situ Gelatin Zymography.- Method for Determining Gelatinolytic Activity in Tissue Extracts: Real-Time Gelatin Zymography.- In Vitro Spheroid Sprouting Assay of Angiogenesis.- Matrigel Plug Assay for In Vivo Evaluation of Angiogenesis.- Exosomes from Cell Culture Conditioned Medium: Isolation by Ultracentrifugation and Characterization.- Preparation and Characterisation of Rich in Extracellular Matrix Tissue Surrogates Using the Principles of Macromolecular Crowding.- Novel Approaches for Extracellular Matrix Targeting in Disease Treatment.