Um unsere Webseiten für Sie optimal zu gestalten und fortlaufend zu verbessern, verwenden wir Cookies. Durch Bestätigen des Buttons »Akzeptieren« stimmen Sie der Verwendung zu. Über den Button »Einstellungen« können Sie auswählen, welche Cookies Sie zulassen wollen.

AkzeptierenEinstellungen
Alpha-Synuclein -

Alpha-Synuclein

Methods and Protocols

Tim Bartels (Herausgeber)

Buch | Hardcover
289 Seiten
2019 | 1st ed. 2019
Humana Press Inc. (Verlag)
978-1-4939-9123-5 (ISBN)
213,99 inkl. MwSt
This volume provides the reader with detailed protocols that describe a variety of in vitro and in vivo techniques that study reductionist systems and human samples. The methods covered in this book explore a broad selection of topics such as functional aspects of alpha-synuclein biology, its lipid-interactions and misfolding, and functional deficits in cells and mice. Chapters also cover topics such as kinetic measurements of endocytosis and exocytosis in cultured neurons; electrochemiluminescence-based detection; yeast-based screens to target alpha-synuclein toxicity; mass-spectrometry of alpha-synuclein in rat cortical neurons; and expression and purification of untagged a-synuclein. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Alpha-Synuclein: Methods and Protocols is a valuable reference guide that aids researchers working on topics related to alpha-synuclein and its various aspects.

Assays for Neuronal Defects Caused by Early Formation of α-Synuclein Inclusions in Primary Cultured Neurons.- Immunohistochemical Detection of Alpha-Synuclein in Unfixed Human Brain Tissue.- Kinetic Measurements of Endocytosis and Exocytosis in Cultured Neurons.- Detection of Misfolded α-Synuclein Aggregates in Cerebrospinal Fluid by the Protein Misfolding Cyclic Amplification Platform.- Stereotaxic Targeting of Alpha-Synuclein Pathology in Mouse Brain using Pre-Formed Fibrils.- Quantification of Alpha-Synuclein in Biological Fluids by Electrochemiluminescence-Based Detection.- Alpha-Synuclein Proximity Ligation Assay (AS-PLA) in Brain Sections to Probe for Alpha-Synuclein Oligomers.- Studying α-Synuclein Conformation by Intact-Cell Crosslinking.- C. elegans as a Model for Synucleinopathies and Other Neurodegenerative Diseases: Tools and Techniques.- Recombinant Expression and Purification of N-Acetylated Alpha-Synuclein.- CircularDichroism and Isothermal Titration Calorimetry to Study the Interaction of α-Synuclein with Membranes.- Yeast-Based Screens to Target Alpha-Synuclein Toxicity.- Probing Structural Changes in Alpha Synuclein by Nuclear Magnetic Resonance Spectroscopy.- Measuring Mitochondrial Dysfunction Caused by Soluble α-Synuclein Oligomers.- Monitoring α-Synuclein Proteotoxicity in Drosophila Models.- Detection of α-Synuclein in Biological Samples using Mass Spectrometry.- Single-Molecule FRET Detection of Early-Stage Conformations in α-Synuclein Aggregation.- In Situ Peroxidase Labelling and Mass-Spectrometry of Alpha-Synuclein in Rat Cortical Neurons.- Probing Alpha-Synuclein Conformations by Electron Paramagnetic Resonance (EPR) Spectroscopy.- Expression and Purification of Untagged α-Synuclein.- Generation and Analysis of Viral Vector Mediated Rodent Models for Parkinson’s Disease.

Erscheinungsdatum
Reihe/Serie Methods in Molecular Biology ; 1948
Zusatzinfo 55 Illustrations, color; 11 Illustrations, black and white; XII, 289 p. 66 illus., 55 illus. in color.
Verlagsort Totowa, NJ
Sprache englisch
Maße 178 x 254 mm
Themenwelt Naturwissenschaften Biologie Biochemie
Naturwissenschaften Biologie Humanbiologie
Naturwissenschaften Biologie Mikrobiologie / Immunologie
Naturwissenschaften Biologie Zoologie
Schlagworte Amyloid Formation • endocytosis • Exocytosis • protein-protein interaction • Rodent models • Toxicity
ISBN-10 1-4939-9123-X / 149399123X
ISBN-13 978-1-4939-9123-5 / 9781493991235
Zustand Neuware
Informationen gemäß Produktsicherheitsverordnung (GPSR)
Haben Sie eine Frage zum Produkt?
Mehr entdecken
aus dem Bereich
Die Biochemie und industrielle Nutzung von Naturstoffen

von Oliver Kayser; Nils J. H. Averesch

Buch | Softcover (2024)
Springer Spektrum (Verlag)
49,99

von Reinhard Matissek; Andreas Hahn

Buch (2024)
Springer Spektrum (Verlag)
79,99