Matrix Metalloproteases

Expression and Purification of Matrix Metalloproteinases in Escherichia coli.- Expression and Purification of a Matrix Metalloprotease Transmembrane Domain in Escherichia coli.- Heterologous Expression of the Astacin Protease Meprin b in Pichia pastoris.- Structural Studies of Matrix Metalloproteinase by X-Ray Diffraction.- Mapping Lipid Bilayer Recognition Sites of Metalloproteinases and other Prospective Peripheral Membrane Proteins.- Using Small Angle X-ray Scattering (SAXS) to Characterise the Solution Conformation and Flexibility of Matrix Metalloproteinases (MMPs).- Molecular Dynamics Studies of Matrix Metalloproteases.- Determining the Substrate Specificity of Matrix Metalloproteases using Fluorogenic Peptide Substrates.- Time-resolved Analysis of Matrix Metalloproteinase Substrates in Complex Samples.- Identification of Protease Cleavage Sites by Charge-Based Enrichment of Protein N-termini.- Mapping theSubstrate Recognition Landscapes of Metalloproteases using Comprehensive Mutagenesis.- Detection of Matrix Metalloproteinases by Zymography.- Imaging Matrix Metalloproteases in Spontaneous Colon Tumors:  Validation by Correlation with Histopathology.- Virtual High-throughput Screening for Matrix Metalloproteinase Inhibitors.- Computational Approaches to Matrix Metalloprotease Drug Design.- A Simple, Adaptable Blood-Brain Barrier Cell Model for Screening Matrix Metalloproteinase Inhibitor Functionality.- Matrix Metalloproteases as Biomarkers of Disease.