T-Cell Motility

An Introduction to LFA-1/ICAM-1 Interactions in T-Cell Motility.- Isolation of Human Peripheral Blood T Lymphocytes.- A Laboratory Model to Study T-Cell Motility.- Quantification of T-Cell Migratory Phenotypes using High Content Analysis.- Live Cell Imaging and Analysis to Capture T-Cell Motility in Real-Time.- Three-Dimensional Structured Illumination Microscopy (3D-SIM) to Dissect Signalling Cross-Talks in Motile T-Cells.- Real-Time Impedance-Based Detection of LFA-1-Stimulated T-Cell Transwell Chemotaxis.- Quantitative Real-Time PCR for Evaluating Transcriptional Changes in T-Lymphocytes.- GapmeR-Mediated Gene Silencing in Motile T-Cells.- Live Imaging of Resident T-Cell Migration in Human Lymphoid Tissue Slices using Confocal Microscopy.- Phosphoprotein Enrichment for Protein Analysis in Motile T-Lymphocytes.- Immunometabolomic Phenotyping of Motile T-Cells.- Profiling Activity of Cellular Kinases in Migrating T-Cells.- Biotinylation Assay to Determine LFA-1 Recycling in Motile T-Lymphocytes.- A Protocol to Study T-Cell Signalling in an Immune Synapse by Microscopy.- Enzyme-Linked Immunosorbent Assay for T-Cell Dependent Immunogenicity Assessment of Therapeutic Peptides.- Modified Intravital Microscopy to Assess Vascular Health and T-Cell Motility.- Computational Analysis of Protein-Protein Interactions in Motile T-Cells.