Calcium Signalling

High-Throughput Screening using Photoluminescence Probe to Measure Intracellular Calcium Levels.- Exploiting Cameleon Probes to Investigate Organelles Ca2+ Handling.- Measuring Ca2+ Levels in Subcellular Compartments with Genetically-Encoded GFP-Based Indicators.- Methods to Measure Intracellular Ca2+ Concentration using Ca2+-Sensitive Dyes.- MCU Regulation in Lipid Bilayer and Electrophysiological Recording.- Electrophysiological Characterization of Calcium-Permeable Channels using Planar Lipid Bilayer.- Patch Clamp Analysis of the Mitochondrial Calcium Uniporter.- In Vivo Light Sheet Fluorescence Microscopy of Calcium Oscillations in Arabidopsis thaliana.- Ex Vivo Measurements of Ca2+ Transients in Intracellular Compartments of Skeletal Muscle Fibres by Means of Genetically Encoded Probes.- Imaging Intracellular Ca2+ in Cardiomyocytes with Genetically Encoded Fluorescent Probes.- In Vivo Monitoring of Ca2+ Uptake into Subcellular Compartments of Mouse Skeletal Muscle.- TRPML1/TFEB-Dependent Regulation of Lysosomal Exocytosis.- Ca2+-Dependent Regulation of TFEB and Lysosomal Function.- Employing Transgenic Parasite Strains to Study the Ca2+ Dynamics in the Human Malaria Parasite Plasmodium falciparum.- Calcium Imaging of Stored Operated Calcium (Ca2+) Entry (SOCE) in HEK293 Cells using Fura-2.- Optogenetic Interneuron Stimulation and Calcium Imaging in Astrocytes.- Measuring Calcium and ROS by Genetically Encoded Protein Sensors and Fluorescent Dyes.- Assessing Calcium-Stimulated Mitochondrial Bioenergetics using the Seahorse XF96 Analyzer.- Determination of ATP, ADP, AMP Levels by Reversed-Phase High Performance Liquid Chromatography in Cultured Cells.- Purification of Functional F-ATP Synthase from Blue Native PAGE.