Vertebrate Embryogenesis

Culture and Host Transfer of Xenopus Oocytes for Maternal mRNA Depletion and Genome Editing Experiments.- Immunofluorescence of Microtubule Assemblies in Amphibian Oocytes and Early Embryos.- Assaying NanoLuc Luciferase Activity from mRNA-Injected Xenopus Embryos.- Reconstitution of Intracellular Calcium Signaling in Xenopus Egg Extracts.- Membrane Microdomains as Platform to Study Membrane-Associated Events during Oogenesis, Meiotic Maturation, and Fertilization in Xenopus laevis.- Embryological and Genetic Manipulation of Chick Development.- Identifying Protein-DNA and Protein-Protein Interactions in Avian Embryos.- Experimental Manipulation of Ploidy in Zebrafish Embryos and its Application in Genetic Screens.- RNA Tomography for Spatially Resolved Transcriptomics (Tomo-Seq).- Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos.- Ex Utero Culture and Imaging of Mouse Embryos.- Detection of Gene and Protein Expression in MouseEmbryos and Tissue Sections.- Reptile Embryology and Regeneration.- Manipulation of Developmental Function in Turtles with Notes on Alligators.- Methods for Isolating the Balbiani Body/Germ Plasm from Xenopus laevis Oocytes.- Visualizing the Balbiani Body in Zebrafish Oocytes.- Isolation of Zebrafish Balbiani Bodies for Proteomic Analysis.- Generation of Xenopus Haploid, Triploid, and Hybrid Embryos.- Germ Cell Transplantation in Avian Species.- Production of Germ-Line Chimeras in Zebrafish.- A Method for Zebrafish Follicle Transplantation into Recipient Mothers for the Generation of Fertilizable Eggs and Viable Offspring.- Optimized Protocol of Zebrafish Somatic Cell Nuclear Transfer (SCNT).- An Accessible Protocol for the Generation of CRISPR- Cas9 Knockouts Using INDELs in Zebrafish.- Modeling Embryonic Cleavage Patterns.- Laboratory Guidelines for Animal Care.