Hepatitis C Virus Protocols

Overview of Direct Acting Antiviral Drugs and Drug Resistance of Hepatitis C Virus.- Similarities and Differences between HCV Pseudoparticle (HCVpp) and Cell Culture HCV (HCVcc) in the Study of HCV.- Hepatitis C Virus Database and Bioinformatics Analysis Tools in the Virus Pathogen Resource (ViPR).- Establishment of Replication-competent HCV Strain with Minimum Modifications.- Full-length Open Reading Frame Amplification of Hepatitis C Virus.- InFusion Cloning for the Generation of Biologically Relevant HCV Chimeric Molecular Clones.- Production and Purification of Cell Culture Hepatitis C Virus.- Embryonic or Induced Pluripotent Stem Cell-derived Hepatocellular Systems for HCV Culture.- Molecular Identification of Transmitted/Founder Hepatitis C Viruses and their Progeny by Single Genome Sequencing.- A Cell Culture Model for Persistent HCV Infection.- Expression Cloning of Host Factors Required for the HCV Replication Cycle.- Application of Deep Mutational Scanning in Hepatitis C Virus.- Studying the Hepatitis C Virus-induced Epigenetic Signature after Cure with Direct-acting Antivirals.- Measuring Hepatitis C Virus Envelopment by using a Proteinase K Protection Assay.- Membrane Fusion Assays for Studying Entry Hepatitis C Virus into Cells.- Functional Characterization of Apolipoproteins in the HCV Life Cycle.- Live Cell Imaging of Hepatitis C Virus Trafficking in Hepatocytes.- Cloning and Analysis of Authentic Patient-derived HCV E1/E2 Glycoproteins.- Simultaneous Quantification of Hepatitis C virus Envelope Glycoproteins E1 and E2 by Dual-color Fluorescence Immunoblot Analysis.- Overcoming Challenges of Hepatitis C Virus Envelope Glycoprotein Production in Mammalian Cells.- Monitoring of Interferon Response Triggered by Cells Infected by Hepatitis C Virus or Other Viruses upon Cell-cell Contact.- Complement Regulation and Immune Evasion by Hepatitis C Virus.- Direct ex vivo Functional Analysis of HCV-specific T Cells.- ­Simultaneous Analysis of HCV-specific CD4+ and CD8+ T Cells by Multi-color Flow Cytometry.- Probing the Antigenicity of HCV Envelope Glycoproteins by Phage Display Antibody Technology. -Isolation of HCV Neutralizing Antibodies by Yeast Display.- Detection of Antibodies to HCV E1E2 by Lectin-Capture ELISA.- In vitro Neutralization Assay using Cultured Hepatitis C Virus.- Standardized Method for the Study of Antibody Neutralization of HCV pseudoparticles (HCVpp).- Detection of Antigen-specific CD8+ T Cells in the Livers of HCV Core Transgenic Mice.- Mice with Chimeric Human Livers and Their Applications.- Mouse Models for Studying HCV Vaccines and Therapeutic Antibodies.