Bacterial Protein Secretion Systems
Humana Press Inc. (Verlag)
978-1-4939-8374-2 (ISBN)
Authoritative and cutting-edge, Bacterial Protein Secretion Systems: Methods and Protocol aims to provide techniques that are not restricted to the study of secretion systems but arealso of specific interest for any researcher interested on multi-protein complexes of the bacterial cell envelope.
Identification of Protein Secretion Systems in Bacterial Genomes using MacSyFinder.- Protein Sorting Prediction .- Cell Fractionation.- Defining Lipoprotein Localisation by Fluorescence Microscopy.- Components Sub-cellular Localization: Identification of Lipoproteins using Globomycin and Radioactive Palmitate.- Defining Membrane Protein Localization by Isopycnic Density Gradients.- Components Sub-cellular Localisation: Cell Surface Exposure.- Probing Inner Membrane Protein Topology by Proteolysis.- Mapping of Membrane Protein Topology by Substituted Cysteine Accessibility Method (SCAMTM).- Defining Membrane Protein Topology using pho-lac Reporter Fusions.- In vivo and in vitro Protein-peptidoglycan Interactions.- Measure of Peptidoglycan Hydrolase Activity.- Protein-protein Interaction: Bacterial Two-Hybrid.- Protein-protein Interactions: Yeast two-hybrid.- Protein-protein Interactions: Cytology Two-hybrid.- Fusion Reporter Approaches to Monitor Transmembrane Helix Interactions in Bacterial Membranes.- Protein-protein Interactions: Co-immunoprecipitation.- Protein-protein Interaction: Tandem Affinity Purification in Bacteria.- Site-directed and Time-resolved Photocrosslinking in Cells Metabolically Labeled With Radioisotopes.- Protein-protein Interactions: Pull-down Assays.- Protein-protein interactions: Surface Plasmon Resonance.- Assessing Energy-dependent Protein Conformational Changes in the TonB System.- Defining Assembly Pathways by Fluorescence Microscopy.- Large Complexes: Cloning Strategy, Production, and Purification.- Shearing and Enrichment of Extracellular Type IV Pili.- Blue Native PAGE Analysis of Bacterial Secretion Complexes.- In situ Imaging of Bacterial Secretion Systems by Electron Cryo-tomography.- Structural Analysis of Protein Complexes by Cryo Electron Microscopy.- Bacterial Filamentous Appendages Investigated by Solid-state NMR Spectroscopy.- Identification of Effectors: Precipitation of Supernatant Material.- Energy Requirements for Protein Secretion via the Flagellar type-III Secretion System.- Screening for Secretion of the Type VI Secretion Protein Hcp by Enzyme-Linked Immunosorbent Assay and Colony Blot.- Effector Translocation: Cya Reporter Assay.- Monitoring Effector Translocation with the TEM-1 Beta-lactamase Reporter System.- Effector Translocation Assay: Differential Solubilization.- Quantitative Determination of Anti-bacterial Activity during Bacterial Co-culture.
Erscheinungsdatum | 24.09.2018 |
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Reihe/Serie | Methods in Molecular Biology ; 1615 |
Zusatzinfo | 70 Illustrations, color; 26 Illustrations, black and white; XV, 528 p. 96 illus., 70 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Allgemeines / Lexika |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
ISBN-10 | 1-4939-8374-1 / 1493983741 |
ISBN-13 | 978-1-4939-8374-2 / 9781493983742 |
Zustand | Neuware |
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