3D Cell Culture -

3D Cell Culture

Methods and Protocols

Zuzana Koledova (Herausgeber)

Buch | Softcover
452 Seiten
2018 | Softcover reprint of the original 1st ed. 2017
Humana Press Inc. (Verlag)
978-1-4939-8370-4 (ISBN)
133,74 inkl. MwSt
This book provides an overview of established 3D cell culture assays from leaders in the field. Their contributions cover a wide spectrum of techniques and approaches for 3D cell culture, from organoid cultures through organotypic models to microfluidic approaches and emerging 3D bioprinting techniques, which are used in developmental, stem cell, cancer, and pharmacological studies, among many others. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Comprehensive and cutting-edge, 3D Cell Culture: Methods and Protocols aims to inspire researchers to develop novel 3D cell culture techniques according to their specific scientific needs and interests, leading to a new generation of physiologically relevant and realistic 3D cell cultures.

Chapter 15 of this book is available open access under a CC BY 4.0 license.

3D Cell Culture: An Introduction.- Preparation of Decellularized Biological Scaffolds for 3D Cell Culture.- 3D Cell Culture in Interpenetrating Networks of Alginate and rBM Matrix.- Hydrogel-Based In Vitro Models of Tumor Angiogenesis.- Generation of Induced Pluripotent Stem Cells in Defined Three-Dimensional PEG Hydrogels.- Calcium Phosphate Foams: Potential Scaffolds for Bone Tissue Modelling in Three-Dimensions.oids.- 3d="" fluid-dynamic="" models="" multiple="" myeloma.- preparation="" three-dimensional="" full="" thickness="" skin="" equivalent.- analysis="" breast="" cell="" invasion="" using="" an="" system.- 3d="" the="" brain="" parenchyma="" metastasis="" interface="" metastasis.- 3d="" neural="" in="" dual="" hydrogel="" systems.- 3d="" micro-patterned="" hydrogels="" prepared="" by="" photomask,="" micro-needle,="" or="" soft="" lithography="" techniques.- 3d="" niche="" engineering="" via="" two-photon="" laser="" polymerization.- microfluidic-based="" generation="" collagen="" spheres="" investigate="" multicellular="" spheroid="" invasion.- high-throughput="" sphere="" formation="" culture.- high-throughput="" tumor="" recyclable="" microfluidic="" platform.- high-throughput="" platform="" mesenchymal="" anastomosed="" microvascular="" network="" living="" capillary="" networks="" and="" endothelial="" cell-lined="" channels.- human="" small="" airway-on-a-chip="" protocol.- microfluidic="" bioprinting="" heterogeneous="" tissue="" constructs.- bioprinting="" decellularized="" extracellular="" matrix="" bioink.- bioprinting="" cartilage="" peg="" hydrogel.- real-time="" cycle="" imaging="" melanoma.- revealing="" ultrastructure="" morphology="" spheroids="" electron="" microscopy.- quantitative="" phenotypic="" image="" analysis="" cultures.

Erscheinungsdatum
Reihe/Serie Methods in Molecular Biology ; 1612
Zusatzinfo 99 Illustrations, color; 15 Illustrations, black and white; XVI, 452 p. 114 illus., 99 illus. in color.
Verlagsort Totowa, NJ
Sprache englisch
Maße 178 x 254 mm
Themenwelt Naturwissenschaften Biologie Genetik / Molekularbiologie
Naturwissenschaften Biologie Mikrobiologie / Immunologie
Naturwissenschaften Biologie Zellbiologie
Schlagworte 3D Organoids • Bioprinting • Hydrogels • microfluidics • Micropatterning • Organotypic cultures • Scaffolds • Three-dimensional cell culture
ISBN-10 1-4939-8370-9 / 1493983709
ISBN-13 978-1-4939-8370-4 / 9781493983704
Zustand Neuware
Haben Sie eine Frage zum Produkt?
Mehr entdecken
aus dem Bereich
50 Meilensteine der Genetik

von Jonathan Weitzman; Matthew Weitzman

Buch | Hardcover (2022)
Librero b.v. (Verlag)
9,95