Heterologous Protein Production in CHO Cells

Strategies and Considerations for Improving Expression of ‘Difficult to Express’ Proteins in CHO Cells.- Glycoengineering of CHO Cells to Improve Product Quality.- Large-Scale Transient Transfection of Chinese Hamster Ovary Cells in Suspension.- Cloning of Single-Chain Antibody Variants by Overlap-Extension PCR for Evaluation of Antibody Expression in Transient Gene Expression.- Anti-Apoptosis Engineering for Improved Protein Production from CHO Cells.- Conditional Knockdown of Endogenous MicroRNAs in CHO Cells using TET-ON-SanDI Sponge Vectors.- Application of CRISPR/Cas9 Genome Editing to Improve Recombinant Protein Production in CHO Cells.- Improved CHO Cell Line Stability and Recombinant Protein Expression During Long-Term Culture.- Selection of High-Producing Clones using FACS for CHO Cell Line Development.- The ‘Omics Revolution in CHO Biology: Roadmap to Improved CHO Productivity.- A Bioinformatics Pipeline for the Identification of CHO Cell Differential Gene Expression from RNA-Seq Data.- Filter-Aided Sample Preparation (FASP) for Improved Proteome Analysis of Recombinant Chinese Hamster Ovary Cells.- Phosphopeptide Enrichment and LC-MS/MS Analysis to Study the Phosphoproteome of Recombinant Chinese Hamster Ovary Cells.- Engineer Medium and Feed for Modulating N-Glycosylation of Recombinant Protein Production in CHO Cell Culture.- Glycosylation Analysis of Therapeutic Glycoproteins Produced in CHO Cells.- Characterization of Host Cell Proteins (HCPs) in CHO Cell Bioprocesses.