Skeletal Muscle Regeneration in the Mouse -

Skeletal Muscle Regeneration in the Mouse

Methods and Protocols

Michael Kyba (Herausgeber)

Buch | Softcover
346 Seiten
2018 | Softcover reprint of the original 1st ed. 2016
Springer-Verlag New York Inc.
978-1-4939-8145-8 (ISBN)
117,69 inkl. MwSt
Chapters address the three major areas of study: provoking regeneration by inducing damage to muscle, analyzing theprogenitor cells of skeletal muscle, and quantifying overall muscle function.
This volume focuses
on the cell biology and physiology of skeletal muscle regeneration. This Book
is a collection of classic and cutting edge protocols optimized for mice, but
in most cases adaptable to rat or other mammalian models, that will allow an
investigator to develop and implement a research study on skeletal muscle
regeneration. Chapters address the three major areas of study: provoking regeneration by inducing damage to muscle, analyzing the
progenitor cells of skeletal muscle, and quantifying overall muscle function. Subjects discussed include: inducing skeletal muscle injury by
eccentric contraction; volumetric muscle loss; single myofiber isolation and
culture; satellite cell transplantation; muscle clearing for whole mount
immunostaining; luciferase tracking of muscle stem cells; mitochondrial
and mitophagy flux analysis; in vivo assessment of muscle contractility;
force measurements on single isolated myofibers; and analysis of aerobic
respiration in intact skeletal muscle tissue by microplate respirometry. Written in the highly
successful Methods in Molecular Biology series format,
chapters include introductions to each respective topic, lists of the necessary
materials and reagents, step-by-step, readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and
practical, Skeletal Muscle Regeneration in the Mouse: Methods and Protocols is an essential laboratory reference for research in
skeletal muscle growth, damage, repair, degeneration, and regenerative therapy
in the mouse model system.

Michael Kyba, Ph.D. Associate Professor and Lillehei Endowed Scholar, Lillehei Heart Institute NHH4.126, University of Minnesota, SE Minneapolis, MN 55455, USA

Eccentric
Contraction-Induced Muscle Injury: Reproducible, Quantitative, Physiological Models
to Impair Skeletal Muscle’s Capacity to Generate Force.- Volumetric
Muscle Loss.- Freeze Injury of the Tibialis Anterior
Muscle.- Synergist Ablation
as a Rodent Model to Study Satellite Cell Dynamics in Adult Skeletal Muscle.- Inducing
and Evaluating Skeletal Muscle Injury by Notexin and Barium Chloride.- Cardiotoxin Induced Injury and
Skeletal Muscle Regeneration.- Fibrosis-Inducing Strategies in Regenerating Dystrophic and Normal Skeletal
Muscle.- Isolation, Cryosection, and Immunostaining
of Skeletal Muscle.- Isolation of Mouse Periocular Tissue
for Histological and Immunostaining Analyses of the Extraocular Muscles and
their Satellite Cells.- Skeletal Muscle Tissue Clearing
for LacZ and Fluorescent Reporters, and Immunofluorescence Staining.- Isolation, Culture, Functional
Assays and Immunofluorescence of Myofiber-Associated Satellite Cells.- Transplantation-Based Quantitative
Assays for Satellite Cell Self-Renewal and Differentiation.- Non-Invasive Tracking of Quiescent
and Activated MuSC Engraftment Dynamics In Vivo.- Myogenic Progenitors from Mouse Pluripotent
Stem Cells for Muscle Regeneration.- Assaying Human Myogenic Progenitor
Cell Activity by Reconstitution of Muscle Fibers and Satellite Cells in Immunodeficient
Mice.- Methods for Mitochondria and Mitophagy
Flux Analyses in Stem Cells of Resting and Regenerating Skeletal Muscle.- Identification, Isolation, and
Characterization of Mesenchymal Progenitors in Mouse and Human Skeletal Muscle.- FACS Fractionation and Differentiation of Skeletal-Muscle
Resident Multipotent Tie2+ Progenitors.- In Vitro Assays to Determine
Skeletal Muscle Physiologic Function.- In Vivo Assessment of Muscle
Contractility in Animal Studies.- Functional Measurement of
Respiratory Muscle Motor Behaviors Using Transdiaphragmatic Pressure.- Assessment of the Contractile Properties
of Permeabilized Skeletal Muscle Fibers.- Analysis of Aerobic Respiration
in Intact Skeletal Muscle Tissue by Microplate-Based Respirometry.

Erscheinungsdatum
Reihe/Serie Methods in Molecular Biology ; 1460
Zusatzinfo 87 Illustrations, color; 10 Illustrations, black and white; XII, 346 p. 97 illus., 87 illus. in color.
Verlagsort New York
Sprache englisch
Maße 178 x 254 mm
Themenwelt Medizin / Pharmazie Studium
Naturwissenschaften Biologie Genetik / Molekularbiologie
Schlagworte Degeneration • immunofluorescence staining • muscle Injury • Regenerative therapy • satellite cells • skeletal muscle growth • skeletal muscle progenitor cells • Tissue repair
ISBN-10 1-4939-8145-5 / 1493981455
ISBN-13 978-1-4939-8145-8 / 9781493981458
Zustand Neuware
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