Proteostasis

UPS Activation in the Battle against Aging and Aggregation-Related
Diseases: An Extended Review.- Review
And Literature Mining On Proteostasis Factors’ And Cancer.- Combining Zebrafish and Mouse Models to Test the
Function of Deubiquitinating Enzyme (Dubs) Genes in Development:  Role Of Usp45 in the Retina.-  Immunodepletion and Immunopurification as Approaches
for CSN Research.-  Studying Protein Ubiquitylation in Yeast.- Strategies to Detect Endogenous Ubiquitination of a
Target Mammalian Protein.- In Vitro Ubiquitination – Self-Ubiquitination, Chain
Formation, and Substrate Ubiquitination Assays.- Isolation of the Ubiquitin-Proteome From Tumour Cell
Lines and Primary Cells Using Tubes.- Tubes-Mass Spectrometry for Identification and
Analysis of the Ubiquitin-Proteome.- Isolation of Ubiquitinated Proteins to High
Purity  from In Vivo Samples.-  Method for the Purification of Endogenous Unanchored
Polyubiquitin Chains.- Fluorescent Tools for In Vivo Studies on
the Ubiquitin-Proteasome System.- Bimolecular Fluorescence Complementation to Assay
the Interactions of Ubiquitylation Enzymes in Living Yeast Cells.- Monitoring Ubiquitin-Coated Bacteria Via Confocal
Microscopy.- Detection and Analysis of Cell Cycle-Associated
Apc/C-Mediated Cellular Ubiquitylation In Vitro and In Vivo.-  Detection and Analysis Of Sumoylation Substrates In
Vitro and In Vivo. - Detection of Protein-Protein Interactions and
Post-Translational Modifications using the Proximity Ligation Assay:
Application to the Study of the Sumo Pathway.- Dissecting of Sumo Dynamics by Mass Spectrometry.- Isolation of Lysosomes from Mammalian Tissues and
Cultured Cells.- Analysis Of Relevant Parameters For Autophagic Flux
Using Hela Cells Expressing Egfp-Lc3.-  Analysis of Protein Oligomeric Species by Sucrose
Gradients.- Analysis of
Protein Oligomerization by Electrophoresis.-Blot-Ms of Carbonylated Proteins: A Tool
to Identify Oxidized Proteins.- Quantitation of
Protein Translation Rate In Vivo with Bioorthogonal Click-Chemistry.- A Simple Protocol for High Efficiency Protein
Isolation after RNA Isolation from Mouse Thyroid and Other Very Small Tissue
Samples.-  Monitoring Target Engagement Of Deubiquitylating
Enzymes Using Activity Probes:  Past,
Present, and Future.- Activity Based Profiling of Deubiquitylating Enzymes
and Inhibitors in Animal Tissues.- High-Throughput Sirna Screening Applied to the
Ubiquitin-Proteasome System.-  High-Throughput Yeast-Based Reporter Assay to
Identify Compounds with Anti-Inflammatory Potential.- Using
Alphascreen ® to Identify Small Molecule Inhibitors Targeting a
Conserved Host-Pathogen Interaction.- Global MS-Based Proteomics Drug Profiling.