Peroxisomes

Isolation of Peroxisomes from Rat Liver and Cultured Hepatoma Cells by Density Gradient Centrifugation.- Isolation of Peroxisomes from Mouse Brain Using a Continuous Nycodenz Gradient: A Comparison to the Isolation of Liver and Kidney Peroxisomes.- Determining the Topology of Peroxisomal Proteins Using Protease Protection Assays.- Isolation of Native Soluble and Membrane-Bound Protein Complexes from Yeast Saccharomyces Cerevisiae.- Method for Measurement of Peroxisomal Very Long-Chain Fatty Acid Beta-Oxidation and de novo C26:0 Synthesis Activity in Living Cells Using Stable-Isotope Labelled Docosanoic Acid.- Analysis of Plasmalogen Synthesis in Cultured Cells.- Transfection of Primary Human Skin Fibroblasts for Peroxisomal Studies.- siRNA-Mediated Silencing of Peroxisomal Genes in Mammalian Cells.- Dual Reporter Systems for the Analysis of Translational Readthrough in Mammals.- Cytochemical Detection of Peroxisomes in Light and Electron Microscopy with 3,3’-diaminobenzidine.- Cryo-Immuno Electron Microscopy of Peroxisomal Marker Proteins.- Detection and Immunolabelling of Peroxisomal Proteins.- Labelling of Peroxisomes for Live Cell Imaging in the Filamentous Fungus Ustilago Maydis.- Quantitative Monitoring of Subcellular Redox Dynamics in Living Mammalian Cells Using RoGFP2-based Probes.- KillerRed as a Tool to Study the Cellular Responses to Peroxisome-Derived Oxidative Stress.- Determination of Peroxisomal pH in Living Mammalian Cells Using pHRed.- In Cellulo Approaches to Study Peroxisomal Protein Import – Yeast Immunofluorescence Microscopy.- Blue Native Page: Applications to Study Peroxisome Biogenesis.- In Vitro PMP Import Analysis Using Cell-Free Synthesized PMP and Isolated Peroxisomes.- Peroxisomal Membrane and Matrix Protein Import Using a Semi-Intact Mammalian Cell System.- The Use of Glycosylation Tags as Reporters for Protein Entry into the Endoplasmic Reticulum in Yeast and Mammalian Cells.- Detection of Ubiquitinated Peroxisomal Proteins in Yeast.- Assessing Pexophagy in Mammalian Cells.- Experimental Systems to Study Yeast Pexophagy.- Flow Cytometric Analysis of the Expression Pattern of Peroxisomal Proteins, Abcd1, Abcd2, and Abcd3 in BV-2 Murine Microglial Cells.- Study of Peroxisomal Protein Phosphorylation by Functional Proteomics.- Analysis of Peroxisomal β-Oxidation during Storage Oil Mobilization in Arabidopsis Thaliana Seedlings.- Peroxisome Mini-Libraries - Systematic Approaches to Study Peroxisomes Made Easy.- Generation of Peroxisome-Deficient Somatic Animal Cell Mutants.- Clinical and Laboratory Diagnosis of Peroxisomal Disorders.