Phagocytosis and Phagosomes
Humana Press Inc. (Verlag)
978-1-4939-8241-7 (ISBN)
Authoritative and cutting-edge, Phagocytosis and Phagosomes: Methods and Protocols aims to be an important resource for both experts in the field and for those investigators delving into phagocytosis and phagosome maturation for the first time.
Phagocytosis: Hungry, Hungry Cells.- Analysis of Human and Mouse Neutrophil Phagocytosis by Flow Cytometry.- Quantitative Efferocytosis Assays.- Quantifying Phagocytosis by Immunofluorescence and Microscopy.- Single Cell Analysis of Phagocytosis, Phagosome Maturation, Phagolysosomal Leakage and Cell Death Following Exposure of Macrophages to Silica Particles.- Quantitative Live-cell Fluorescence Microscopy during Phagocytosis.- Intracellular Manipulation of Phagosomal Transport and Maturation Using Magnetic Tweezers.- Quantitative Immunofluorescence to Study Phagosome Maturation.- Using Fluorescence Resonance Energy Transfer-Based Biosensors to Probe Rho Gtpase Activation during Phagocytosis.- Analysis of LC3-associated Phagocytosis and Antigen Presentation.- Quantitative Spatio-Temporal Analysis of Phagosome Maturation in Live Cells.- Measuring Phagosomal pH by Fluorescence Microscopy.- Image-Based Analysis of Phagocytosis – Measuring Engulfment and Internalization.- Fluorometric Approaches to Measuring Reductive and Oxidative Events in Phagosomes.- Simultaneous Analysis of Multiple Lumenal Parameters of Individual Phagosomes Using High-Content Imaging.- Salmonella Enterica Internalization and Vacuole Lysis in Epithelial Cells.- Bacterial Binding, Phagocytosis and Killing: Measurements Using Colony Forming Units.- Filamentous Bacteria as Targets to Study Phagocytosis.- Growing and Handling of Mycobacterium Tuberculosis for Macrophage Infection Assays.- Mycobacterium Tuberculosis: Readouts of Bacterial Fitness and The Environment within the Phagosome.- Using Flow Cytometry to Analyse Cryptococcus Infection in Macrophages.>
Erscheinungsdatum | 18.08.2018 |
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Reihe/Serie | Methods in Molecular Biology ; 1519 |
Zusatzinfo | 24 Illustrations, color; 33 Illustrations, black and white; XII, 360 p. 57 illus., 24 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie |
Schlagworte | Cell fractionation • Pathogens • phagocytes • phagosome maturation • Proteomics • Western blotting |
ISBN-10 | 1-4939-8241-9 / 1493982419 |
ISBN-13 | 978-1-4939-8241-7 / 9781493982417 |
Zustand | Neuware |
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