E. coli Gene Expression Protocols

Cold-Inducible Promoters for Heterologous Protein Expression.- Dual-Expression Vectors for Efficient Protein Expression in Both E. coli and Mammalian Cells.- A Dual-Expression Vector Allowing Expression in E. coli and P. pastoris, Including New Modifications.- Purification of Recombinant Proteins from E. coli by Engineered Inteins.- Calmodulin as an Affinity Purification Tag.- Calmodulin-Binding Peptide as a Removable Affinity Tag for Protein Purification.- Maltose-Binding Protein as a Solubility Enhancer.- Thioredoxin and Related Proteins as Multifunctional Fusion Tags for Soluble Expression in E. coli.- Discovery of New Fusion Protein Systems Designed to Enhance Solubility in E. coli.- Assessment of Protein Folding/Solubility in Live Cells.- Improving Heterologous Protein Folding via Molecular Chaperone and Foldase Co-Expression.- High-Throughput Purification of PolyHis-Tagged Recombinant Fusion Proteins.- Co-Expression of Proteins in E. coli Using Dual Expression Vectors.- Small-Molecule Affinity-Based Matrices for Rapid Protein Purification.- Use of tRNA-Supplemented Host Strains for Expression of Heterologous Genes in E. coli.- Screening Peptide/Protein Libraries Fused to the ? Repressor DNA-Binding Domain in E. coli Cells.- Studying Protein-Protein Interactions Using a Bacterial Two-Hybrid System.- Using Bio-Panning of FLITRX Peptide Libraries Displayed on E. coli Cell Surface to Study Protein-Protein Interactions.- Use of Inteins for the In Vivo Production of Stable Cyclic Peptide Libraries in E. coli.- Hyperphage.- Combinatorial Biosynthesis of Novel Carotenoids in E. coli.- Using Transcriptional-Based Systems for In Vivo Enzyme Screening.- Identification of Genes Encoding Secreted Proteins Using Mini-OphoA Mutagenesis.

"...many will find it extremely useful to have this book on hand when trying to achieve high levels of pure, soluble recombinant protein; or incorporating an E. coli expression step into the procedures of functional genomics, proteomics and protein engineering." - Microbiology Today