Type 3 Secretion Systems
Humana Press Inc. (Verlag)
978-1-4939-8260-8 (ISBN)
Practical and comprehensive, Type 3 Secretion Systems: Methods and Protocols is a valuable resource for anyone interested in learning about the fascinating and ever-changing T3S systems.
Introduction to Type III Secretion Systems.- Site-Directed Mutagenesis and its Application in Studying the Interactions of T3S Components.- Blue Native Protein Electrophoresis to Study the T3S System using Yersinia pestis as a Model.- In Vivo Photo-Cross-Linking to Study T3S Interactions Demonstrated using the Yersinia pestis T3S System.- Isolation of Type III Secretion System Needle Complexes by Shearing.- Use of Transcriptional Control to Increase Expression and Secretion of Heterologous Proteins in T3S Systems.- Characterization of Type Three Secretion System Translocator Interactions with Phospholipid Membranes.- Analysis of Type III Secretion System Secreted Proteins.- Fractionation Techniques to Examine Effector Translocation.- Measurement of Effector Protein Translocation using Phosphorylatable Epitope Tags and Phospho-Specific Antibodies.- A TAL-Based Reporter Assay for Monitoring Type III-Dependent Protein Translocationin Xanthomonas.- Subcellular Localization of Pseudomonas syringae pv. tomato Effector Proteins in Plants.- A Method for Characterizing the Type III Secretion System’s Contribution to Pathogenesis: Homologous Recombination to Generate Yersinia pestis Type III Secretion System Mutants.- Detecting Immune Responses to Type III Secretion Systems.- Recombinant Expression and Purification of the Shigella Translocator IpaB.- Expression and Purification of N-Terminally His-Tagged Recombinant Type III Secretion Proteins.- Mouse Immunization with Purified Needle Proteins from Type III Secretion Systems and the Characterization of the Immune Response to these Proteins.- Identification of the Targets of Type III Secretion System Inhibitors.- Detection of Protein Interactions in T3S Systems using Yeast 2-Hybrid Analysis.
Erscheinungsdatum | 16.08.2018 |
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Reihe/Serie | Methods in Molecular Biology ; 1531 |
Zusatzinfo | 23 Illustrations, color; 9 Illustrations, black and white; X, 224 p. 32 illus., 23 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Allgemeines / Lexika |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Schlagworte | effector translocation • heterologous proteins • Immune responses • interactions of T3S components • needle proteins • site-directed mutagenesis |
ISBN-10 | 1-4939-8260-5 / 1493982605 |
ISBN-13 | 978-1-4939-8260-8 / 9781493982608 |
Zustand | Neuware |
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