HDAC/HAT Function Assessment and Inhibitor Development

Detection of Autophagy Induction After HDAC Inhibitor Treatment in Leukemic Cells.- Assessment of HDACi-Induced Protein Cleavage by Caspases.- Assessment of HDACi-Induced Cytotoxicity.- Analysis of HDACi-Induced Changes in Chromosomal Passenger Complex Localization.- Analysis of Histone Deacetylase-Dependent Effects on Cell Migration Using the Stripe Assay.- Using Histone Deacetylase Inhibitors to Analyze the Relevance of HDACs for Translation.- Effects of HDACi on Immunological Functions.- DNA Fiber Spreading Assay to Test HDACi Effects on DNA and Its Replication.- Assessing the Effect of Class I Histone Deacetylase Activity on DNA Double-Strand Break Repair by Homologous Recombination.- Establishment and Characterization of Long-Term Cultures Derived from Primary Acute Myeloid Leukemia Cells for HDAC Inhibitor Research.- Assessing the Histone Deacetylase Activity of SIRT6 in Primary Murine Hepatocytes Via Proximity Ligation Assay.- Purification and Analysis of Male Germ Cells from Adult Mouse Testis.- Generation of Tissue-Specific Mouse Models to Analyze HDAC Functions.- Engineering of Conditional Class I Hdac Knockout Mice and Generation of a Time-Spatial Knockout by a Dual Recombination System.- Xenografting of Cancer Cell Lines for In Vivo Screening of the Therapeutic Potential of HDAC Inhibitors.- Generation of Xenotransplants from Human Cancer Biopsies to Assess Anti-Cancer Activities of HDACi.- Phospho-Flow Analysis of Primary Mouse Cells after HDAC Inhibitor Treatment.- Evaluation of Innate Immune Gene Expression Following HDAC Inhibitor Treatment by High Throughput qPCR and PhosFlow Cytometry.- Assessing HDAC Function in the Regulation of Signal Transducer and Activator of Transcription 5 (STAT5) Activity Using Chromatin Immunoprecipitation (ChIP).- Modulation of STAT1-Driven Transcriptional Activity by Histone Deacetylases.- Proximity Ligation Assay to Quantify Foxp3 Acetylation in Regulatory T Cells.- Testing the Effects of SIAH Ubiquitin E3 Ligases on Lysine Acetyl Transferases.- Assessment of HDACi-Induced Acetylation of Non-Histone Proteins by Mass Spectrometry.- Assessing the Role of Paralog-Specific Sumoylation of HDAC1.- Analysis of Histone Deacetylases Sumoylation by Immunoprecipitation Techniques.- How to Distinguish Between the Activity of HDAC1-3 and HDAC6 with Western Blot.- Evaluating the Effect of HDAC8 Inhibition in Malignant Peripheral Nerve Sheath Tumors.- Analyzing the Impact of Pan- and Class-Specific HDACi on Differentiation-Associated Factors.- Encapsulation of the HDACi Ex527 into Liposomes and Polymer-Based Particles.- Preparation of Histone Deacetylase Inhibitor Vorinostat Loaded Poly d, l-lactide-co-glycolide Polymeric Nanoparticles by Nanoprecipitation Method.- Generation and Assessment of Fusions Between HDACi and TKi.