Split Inteins
Humana Press Inc. (Verlag)
978-1-4939-8204-2 (ISBN)
Cutting-edge and thorough, Split Inteins: Methods and Protocols is a valuable resource that will provide guidance toward possibilities of split intein applications, explore proven and detailed protocols adaptable to various research projects, and inspire new method developments.
Affinity Purification of Proteins in Tag-Free Form: Split-Intein-Mediated Ultra-Rapid Purification (SIRP).- Purification of Microbially Expressed Recombinant Proteins via a Dual ELP Split Intein System.- Intracellular Production of Cyclic Libraries with SICLOPPS.- Recombinant Expression of Cyclotides Using Split Inteins.- Ribosomal Synthesis of Thioether-Bridges Bicyclic Peptides.- Preparation of Semisynthetic Peptides Macrocycles Using Split Inteins.- Semisynthesis of Membrane-Attached Proteins Using Split Inteins.- Protein Chemical Modification Inside Living Cells Using Split Inteins.- Segmental Isotopic Labeling of Proteins for NMR Study Using Intein Technology.- Segmental Isotope Labeling of Insoluble Proteins for Solid-State NMR by Protein Trans-Splicing.- Split-Intein Triggered Protein Hydrogels.- A Recessive Pollination Control System for Wheat-Based on Intein-Mediated Protein Splicing.- Conditional Toxin Splicing Using a Split Intein System.- Photocontrol of the Src Kinase in mammalian Cells with a Photocaged Intein.- LOV2-Controlled Photoactivation of Protein Trans-Splicing.- A Cassette Approach for the Identification of Intein Insertion Sites.- Computational Prediction of New Intein Split Sites.
Erscheinungsdatum | 16.08.2018 |
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Reihe/Serie | Methods in Molecular Biology ; 1495 |
Zusatzinfo | 36 Illustrations, color; 26 Illustrations, black and white; XIV, 270 p. 62 illus., 36 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Schlagworte | Affinity chromatography • bioactive cyclotide • elastin-like peptide tags (ELP) • fluorophores • mutations • peptide macrocycles • protein trans-splicing • split-intein fusion proteins • synthetic intein-extein pieces • toxic enzyme barnase • unnatural amino acid mutagenesis |
ISBN-10 | 1-4939-8204-4 / 1493982044 |
ISBN-13 | 978-1-4939-8204-2 / 9781493982042 |
Zustand | Neuware |
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