Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology

Cell-free translation systems.- Protein synthesis and co-translational folding in cell-free translation systems.- Metabolic burden and stress response.- The cellular response to unfolded proteins in the endoplasmic reticulum.- The effects of recombinant protein expression on the growth and metabolism of mammalian cells.- Mapping stresses in Escherichia coli to improve yield. Examining global gene regulation and « cell conditioning » strategies.- Cellular responses to strong overexpression of recombinant genes in Escherichia coli. DNA relaxation and cell death after induction of ?-glucosidase.- From Vitreoscilla hemoglobin (VHb) to a novel class of growth stimulating hemoglobin proteins.- Genetic stability and gene copy number effects.- Protein mass production in hybridomas and recombinant CHO cells.- Inducible gene copy number amplification for the production of heterologous proteins in Kluyveromyces lactis.- Antibiotic-free plasmid selection and maintenance in Bacteria.- Modelling of segregational plasmid instability of recombinant strain suspension of Escherichia coli.- Transcription, translation, and product formation in E. coli.- Production of optically pure aryl epoxides by recombinant E. coli carrying styrene monooxygenase. A new biocatalyst based on Pseudomonas fluorescens ST genes.- Translational problems associated with the rare arginine CGG in Escherichia coli. Frameshifhting at CGG codons.- Optimisation of the solubility of the recombinant Itk kinase domain in Escherichia coli.- Bacterial senescence and the oxidation paradox.- Metabolic approaches for the optimisation of recombinant fermentation processes.- Control and optimisation of cellular bottlenecks in recombinant protein production.- Expression and fermentation strategies for recombinant proteinproduction in Escherichia coli.- Transcription, translation, and product formation in microbial systems other than E. coli.- Overexpression of a Rhizopus oryzae lipase in Pichia pastoris strains containing multiple copies of the target gene.- Development of a heterologous gene expression system for use in Lactococcus lactis. A novel gram-positive expression system.- Metabolic network analysis for human therapeutic protein productions: effects of the P/O ratio.- Animal cell based expression systems — process optimisation.- Process-orientated metabolic engineering: cell lines with new properties in nutrient exploitation and protein glycosylation.- Influence of the metabolic status of packaging cells on retroviral vector production.- Optimizing the production of recombinant prion protein from CHO cells.- Recombinant protein production by transient transfection of suspension-growing cells.- Comparison of different microbial expression systems.- Production of recombinant human trypsinogen in Escherichia coli and Pichia pastoris. A comparison of expression systems.- Secretion of homologous and heterologous recombinant proteins in Escherichia coli and other gram-negative bacteria by using a new secretion system.- Monitoring of genes that respond to overproduction of insoluble recombinant proteins in Escherichia coli and Bacillus subtilis.- Model supported optimization of fed-batch fermentations for recombinant protein production.- Protease secretion capacity and performance analysis of recombinant Bacillus species.- Authors Index.