Autophagy

Recombinant Expression, Purification and Assembly of p62 Filaments.- Structural Studies of Autophagy-related Proteins.- Structural Studies of Mammalian Autophagy Lipidation Complex.- Structural Studies of Selective Autophagy in Yeast.- Biophysical Studies of LC3 Family Proteins.- Reconstituting Autophagy Initiation from Purified Components.- Cell-free Reconstitution of Autophagic Membrane Formation.- Use of Peptide Arrays for Identification and Characterization of LIR Motifs.- Studying Autophagic Lysosome Reformation in Cells and by an in vitro Reconstitution System.- Formation of Autophagosomes Coincides With Relaxation of Membrane Curvature.- Studies of Receptor-Atg8 Interactions during Selective Autophagy.- Correlative Light and Electron Miscopy of Autophagosomes.- Improved Electron Microscopy (EM) Fixation Methods for Tracking Autophagy-associated Membranes in Cultured Mammalian Cells.- 3-color Simultaneous Live Imaging of Autophagy-related Structures.- CorrelativeLive-cell Imaging and Super-resolution Microscopy of Autophagy.- Methods for Imaging Autophagosome Dynamics in Primary Neurons.- Imaging Autophagy in hiPSC-derived Midbrain Dopaminergic Neuronal Cultures for Parkinson’s Disease Research.- Correlative Light Electron Microscopy to analyze LC3 Proteins in Caenorhabditis elegans embryo.- Imaging Non-canonical Autophagy and LC3-associated Phagocytosis in Cultured Cells.- Measurement of Bulk Autophagy by a Cargo Sequestration Assay.- Methods to Detect Loss of Lysosomal Membrane Integrity.- ­­Monitoring of Autophagy and Cell Volume Regulation in Kidney Epithelial Cells in Response to Fluid Shear Stress.- Identification and Regulation of Multimeric Protein Complexes in Autophagy via SILAC- based Mass Spectrometry Approaches.- Identification and Validation of Novel Autophagy Regulators using an Endogenous Readout siGENOME Screen.- Autophagy Pathway Mapping to Elucidate the Function of Novel Autophagy Regulators Identified by High-throughput Screening.- In vitro Screening Platforms for Identifying Autophagy Modulators in Mammalian Cells.- In vitro Screening Platforms for Identifying Autophagy Modulators in Mammalian Cells.- Methods for the Study of Entotic Cell Death.- MHC Class I Internalization via Autophagy Proteins.- Analysis of Autophagy for Liver Pathogenesis.- Autophagy in 3D In Vitro and Ex Vivo Cancer Models.- Autophagy in Platelets.- Methods to Image Macroautophagy in the Brain In Vivo.- Measuring Nonselective and Selective Autophagy in the Liver.- Measuring Autophagy in Pancreatitis.- Characterization of the “autophagic flux” in Prostate Cancer Tissue Biopsies by LC3A/LAMP2a Immunofluorescence and Confocal Microscopy.- Methods to Determine the Role of Autophagy Proteins in C. elegans Ageing.- Investigating Non-selective Autophagy in Drosophila.- Imaging the Dynamics of Mitophagy in Live Cells.- Triggering Mitophagy with Photosensitizers.- Investigating Mitophagy and Mitochondrial Morphologyin vivo using mito-QC.- Assays to Monitor Mitophagy in Drosophila.- Mitophagy Dynamics in Caenorhabditis elegans.- Methods for Studying Mitophagy in Yeast.- Measuring Anti-bacterial Autophagy.- Quantitative Phosphoproteomics of Selective Autophagy Receptors.- Analysis of Chaperone-mediated Autophagy.- Interactive Autophagy: Monitoring a Novel Form of Selective Autophagy by Macroscopic Observations.