The Nuclear Envelope

Isolation, Proteomic Analysis and Microscopy Confirmation of the Liver Nuclear Envelope Proteome.- Exploring the Protein Composition of the Plant Nuclear Envelope.- High Efficiency Isolation of Nuclear Envelope Protein Complexes from Trypanosomes.- Super Resolution Microscopy of the Nuclear Envelope and Associated Proteins.- Analyses of the Dynamic Properties of Nuclear Lamins by Fluorescence Recovery after Photobleaching (FRAP) and Fluorescence Correlation Spectroscopy (FCS).- Probing Protein Distribution Along the Nuclear Envelope In Vivo by Using Single-Point FRAP.- The Use of 2-Photon FLIM/FRET to Study Protein Interactions during Nuclear Envelope Fusion In Vivo and In Vitro.- Identifying Protein-Protein Associations at the Nuclear Envelope with BioID.- In Situ Detection of Interactions between Nuclear Envelope Proteins and Partners.- Methods for Single-Cell Pulse-Chase Analysis of Nuclear Components.- Analysis of Nuclear Lamina Proteins in Myoblast Differentiation by FunctionalComplementation.- Analysis of Meiotic Telomere Behavior in the Mouse.- Identification and Validation of Putative Nesprin Variants.- Detection of Diverse and High Molecular Weight Nesprin-1 and Nesprin-2 Isoforms Using Western Blotting.- The Use of Polyacrylamide Hydrogels to Study the Effects of Matrix Stiffness on Nuclear Envelope Properties.- Cell Microharpooning to Study Nucleo-Cytoskeletal Coupling.- Wound Healing Assays to Study Mechanisms of Nuclear Movement in Fibroblasts and Myoblasts.- Methods for Assessing Nuclear Rotation and Nuclear Positioning in Developing Skeletal Muscle Cells.- Imaging Approaches to Investigate Myonuclear Positioning in Drosophila.- Mapping Nuclear Lamin-Genome Interactions by Chromatin Immunoprecipitation of Nuclear Lamins.- Lamin ChIP from Chromatin Prepared by Micrococcal Nuclease Digestion.- DamID Analysis of Nuclear Organization in Caenorhabditis elegans.- The Application of DamID to Identify Peripheral Gene Sequences in Differentiated and PrimaryCells.- Visualizing the Spatial Relationship of the Genome with the Nuclear Envelope Using Fluorescence in situ Hybridization.- Visualization of Genomic Loci in Living Cells with a Fluorescent CRISPR/Cas9 System.- Methods to Monitor DNA Repair Defects and Genomic Instability in the Context of a Disrupted Nuclear Lamina.- High Resolution Scanning Electron Microscopy and Immuno-Gold Labeling of the Nuclear Lamina and Nuclear Pore Complex.- An In Vitro Assay to Study Targeting of Membrane Proteins to the Inner Nuclear Membrane.- Nuclear Protein Transport in Digitonin Permeabilized Cells.- Analysis of CRM1-Dependent Nuclear Export in Permeabilized Cells.- SPEED Microscopy and Its Application in Nucleocytoplasmic Transport.