Epitranscriptomics
Humana Press Inc. (Verlag)
978-1-4939-8807-5 (ISBN)
Authoritative and comprehensive, Epitranscriptomics: Methods and Protocols is an important resource for both expert and novice scientists who are interested in learning more aboutthis field.
Bisulfite Sequencing of RNA for Transcriptome-Wide Detection of 5-Methylcytosine.- Single-Molecule Analysis of RNA Dynamics in Living Cells Using Molecular Beacons.- Visualization of Xist Long Non-Coding RNA with Fluorescent CRISPR/CAS9 System.- HAMR: High-Throughput Annotation of Modified Ribonucleotides.- High-Resolution Mapping of N6-Methyladenosine Using m6A Crosslinking Immunoprecipitation Sequencing (m6A-CLIP-seq).- Direct Chemical Biotinylation of RNA 5’-Ends Using a Diazo Reagent.- Identification of Methylated Transcripts Using the TRIBE Approach.- Decoding the Atlas of RNA Modifications from Epitranscriptome Sequencing Data.- Detection of MicroRNA-Mediated Target mRNA Cleavage and 3'-Uridylation in Human Cells by a SLA-RT-PCR Analysis.- Genome-Wide Annotation of circRNAs and Their Alternative Back-Splicing/Splicing with CIRCexplorer Pipeline.- Synthesis and Evaluation of Novel Neamine-Nucleoside Conjugates as Potential Antibiotic Targets for Escherichia coli 16S RibosomalRNA.- Electron Microscope Detection of 5-Methylcytosine on DNA and RNA.- Immuno-Northern Blotting: Detection of Modified RNA Using Gel Separation and Antibodies to Modified Nucleosides.- LncVar:Deciphering Genetic Variations Associated with Long Non-Coding Genes.- Guided Reconstruction of Full-Length Isoforms from Short Reads by CIDANE.- Profiling of N6-Methyladenosine in Zika Virus RNA and Host Cellular mRNA.- Detection and Quantification of Pseudouridine in RNA.- 5-Methylcytosine Analysis by RNA-BisSeq.- RNA Modification Regulatory Genes in DNA Damage.- Dot Blot Analysis for Measuring Global N6-Methyladenosine Modification of RNA.
Erscheinungsdatum | 14.01.2019 |
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Reihe/Serie | Methods in Molecular Biology ; 1870 |
Zusatzinfo | 41 Illustrations, color; 16 Illustrations, black and white; XII, 298 p. 57 illus., 41 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete |
Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik | |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Schlagworte | dsRNAome • ESAT • Eukaryotic cells • immune-northern blotting • post-transcriptional RNA modification • single-cell RNA-sequencing • yeast meiosis |
ISBN-10 | 1-4939-8807-7 / 1493988077 |
ISBN-13 | 978-1-4939-8807-5 / 9781493988075 |
Zustand | Neuware |
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