SNAREs
Humana Press Inc. (Verlag)
978-1-4939-8759-7 (ISBN)
Authoritative and cutting-edge, SNAREs: Methods and Protocols aims to be a valuable tool for all investigators interested in the field.
Molecular Dynamics Simulations of the SNARE Complex.- Mesoscale Computational Modelling of Protein-membrane Interactions based on Continuum Mean-field Theory.- EPR Lineshape Analysis to IOnvestigate the SNARE Folding Intermediates.- Dynamic Light Scattering Analysis to Dissect Intermediates of SNARE-Mediated Membrane Fusion.- Kinetic and Thermodynamic Quantification of Protein Complex Assembly: The Example of SNAREpins.- Single-molecule Optical Tweezers Study of Regulated SNARE Assembly.- Studying Munc18:Syntaxin Interactions using Small-angle Scattering.- Using Force Spectroscopy to Probe Coiled-Coil Assembly and Membrane Fusion.- SNAP25 S-Guanylation and SNARE Complex Formation.- Analysis of the Role of Sec3 in SNARE Assembly and Membrane Fusion.- Use of Microscale Thermophoresis (MST) to Measure Binding Affinities of Components of the Fusion Machinery.- Use of Surface Plasmon Resonance (SPR) to Determine Binding Affinities and Kinetic Parameters Between Components Important in Fusion Machinery.- Determination of Sec18-Lipid Interactions by Liposome Binding Assay.- Using Nanodiscs to Probe Ca2+-dependent Membrane Interaction of Synaptotagmin-1.- Functional reconstitution of Intracellular Vesicle Fusion using Purified SNAREs and Sec1/Munc18 (SM) Proteins.- Assay of Lipid Mixing and Fusion Pore Formation in the Fusion of Yeast Vacuoles.- A nanodisc-cell Fusion Assay with Single Pore Sensitivity and Sub-millisecond Time Resolution.- An In Vitro Assay of trans-SNARE Complex Formation during Yeast Vacuole Fusion using Epitope Tag-free SNAREs.- A Cell-Free Content Mixing Assay for SNARE-Mediated Multivesicular Body-Vacuole Membrane Fusion.- Reconstituted Proteoliposome Fusion Mediated by yeast SNARE-family Proteins.- Real-Time Fluorescence Detection of Calcium Efflux during Vacuolar Membrane Fusion.- Single Molecule Fluorescence Measurement of SNARE-mediated Vesicle Fusion.- Quantifying Intramolecular Protein Conformational Dynamics under Lipid Interaction using smFRET and FCCS.- Visualization of SNARE-Mediated Organelle Membrane Hemifusion By Electron Microscopy.- Studies of the Secretory Machinery Dynamics by Total Internal Reflection Fluorescence Microscopy in Bovine Adrenal Chromaffin Cells.- Imaging SNAP-29 in Drosophila.
Erscheinungsdatum | 26.10.2018 |
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Reihe/Serie | Methods in Molecular Biology ; 1860 |
Zusatzinfo | 66 Illustrations, color; 8 Illustrations, black and white; XIV, 407 p. 74 illus., 66 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
Schlagworte | Eukaryotic cells • Liposomes • membrane trafficking • Post-translational modifications • Reconstituted proteoliposomes |
ISBN-10 | 1-4939-8759-3 / 1493987593 |
ISBN-13 | 978-1-4939-8759-7 / 9781493987597 |
Zustand | Neuware |
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