Microbial Metabolomics

Methods and Protocols

Buch | Hardcover

351 Seiten

2018 | 1st ed. 2019
Humana Press Inc. (Verlag)
978-1-4939-8756-6 (ISBN)

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This detailed volume includes protocols that represent the breadth of microbial metabolomics approaches to both large-scale and small-scale experiments with intention of highlighting techniques that can be used for applications ranging from environmental microbiology to human disease. Utilizing mass spectrometry as their primary measurement tool, the chapters explore microbial metabolomics, metabolism and microbial physiology, metabolite sample preparation, current analytical techniques used to profile primary and secondary metabolites and lipids, as well as establishing data analysis workflows for targeted metabolomics, untargeted metabolomics, analysis of metabolic fluxes, and genome-scale models. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Microbial Metabolomics: Methods and Protocols serves as an ideal reference for both novice and advanced users and can be adapted to similar analytical platforms or customized to suit the needs of the researcher.

Microbial Metabolomics: A General Overview.- Mass Spectrometry-Based Microbial Metabolomics: Techniques, Analysis, and Applications.- Metabolomics: A Microbial Physiology and Metabolism Perspective.- Untargeted Soil Metabolomics Using Liquid Chromatography-Mass Spectrometry and Gas Chromatography-Mass Spectrometry.- Fatty Acid Metabolome Extraction from Mycobacterial Cells for GC-MS Metabolomics Analysis.- Total Metabolome Extraction from Mycobacterial Cells for GC-MS Metabolomics Analysis.- High-Throughput Solid Phase Microextraction-Liquid Chromatography-Mass Spectrometry for Microbial Untargeted Metabolomics.- Targeted Metabolomics of Xylose Fermenting Yeasts Based on Mass Spectrometry.- Exploiting High-Resolution Mass Spectrometry for Targeted Metabolite Quantification and 13C-labeling Metabolism Analysis.- Quantitative Profiling of Endogenous Metabolites Using Hydrophilic Inter-Action Liquid Chromatography-Tandem Mass Spectrometry (HILIC-MS/MS).- Liquid Chromatography and Mass Spectrometry Analysis of Isoprenoid Intermediates in Escherichia coli.- Determining the Mode of Action of Antimalarial Drugs Using Time-Resolved LC-MS-Based Metabolite Profiling.- Use of Liquid Chromatography-Mass Spectrometry-Based Metabolomics to Identify Biomarkers of Tuberculosis.- Metabolomics Analysis of Leishmania by Capillary Electrophoresis and Mass Spectrometry.- A High-Throughput Targeted Metabolomics Workflow for the Detection of 200 Polar Metabolites in Central Carbon Metabolism.- Cluster Analysis of Untargeted Metabolomic Experiments.- Machine Learning in Untargeted Metabolomics Experiments.- Dynamic 13C Labeling of Fast Turnover Metabolites for Analysis of Metabolic Fluxes and Metabolite Channeling.- Genome-Scale 13C Fluxomics Modeling for Metabolic Engineering of Saccharomyces cerevisiae.

Erscheinungsdatum	06.10.2018
Reihe/Serie	Methods in Molecular Biology ; 1859
Zusatzinfo	50 Illustrations, color; 33 Illustrations, black and white; X, 351 p. 83 illus., 50 illus. in color. With online files/update.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Schlagworte	Data Analysis • Mass Spectrometry • metabolic networks • Metabolite sample preparation • Metabolome
ISBN-10	1-4939-8756-9 / 1493987569
ISBN-13	978-1-4939-8756-6 / 9781493987566
Zustand	Neuware