The CRAC Channel

Patch-Clamp Recording of the CRAC Channel Current in STIM-Orai Overexpressing Cells.- Fluorescence-Based Ratiometric Measurement of CRAC Channel Activity in STIM-Orai Overexpressing HEK-293 Cells.- Recording SOCE Activity in Neurons by Patch-Clamp Electrophysiology and Microfluorometric Calcium Imaging.- Mn2+ Quenching Assay for Store-Operated Calcium Entry.- Fluorescence-Based Measurements of Store-Operated Ca2+ Entry in Cancer Cells using Fluo-4 and Confocal Live Cell Imaging.- Fluorescence-Based Measurements of the CRAC Channel Activity in Cell Populations.- Indirect Measurement of CRAC Channel Activity using NFAT Nuclear Translocation by Flow Cytometry in Jurkat Cells.- CRAC Channel  Components Quantitative Expression (In Tissues and Cell Lines) using qPCR.- Western-Blotting and Co-Immunoprecipitation of Endogenous STIM/ORAI and Protein Partners.- Study of Endogenous CRAC Channels in Human Mast Cells using an Adenoviral Delivery System to Transduce Cells with Orai-Targeting shRNAs or with cDNAs Expressing Dominant-Negative Orai Channel Mutations.- Store-Operated Ca2+ Entry in Drosophila Primary Neuron Cultures.- Study of the Endogenous CRAC Channel using shRNA-Mediated Gene Silencing.- Engineered Cross-Linking to Study the Pore Architecture of the CRAC Channel.- Measurement of the CRAC Channel Fast Ca2+-Dependent Inactivation (FCDI).- High Resolution Imaging of STIM/Orai Subcellular Localization using Array Confocal Laser Scanning Microscopy.- Single Channel Single Molecule Detection (SC-SMD) System.