RNA-Protein Complexes and Interactions

Characterization of
RNA-Protein Interactions: Lessons from Two RNA-Binding Proteins, SRSF1 and
SRSF2.- Identification of mRNA-Interacting Factors by MS2-TRAP (MS2-Tagged RNA
Affinity Purification).- Biotin-Streptavidin Affinity Purification of
RNA-Protein Complexes Assembled In Vitro.- Detecting RNA-Protein Interaction
Using End-Labeled Biotinylated RNA Oligonucleotides and Immunoblotting.- Purification
of RNA-Protein Splicing Complexes Using a Tagged Protein from In Vitro Splicing
Reaction Mixture.- Loading of Argonaute Protein with Small Duplex RNA in
Cellular Extracts.- In Vitro Analysis of Ribonucleoprotein Complex Remodeling
and Disassembly.- Single-Turnover Kinetics of Methyl Transfer to tRNA by
Methyltransferases.- Purification and Functional Reconstitution of Box H/ACA
Ribonucleoprotein Particles.- Northwestern Blot Analysis: Detecting RNA-Protein
Interaction After Gel Separation of Protein Mixture.- Measuring mRNA
Translation by Polysome Profiling.- Genome-Wide Profiling of RNA-Protein
Interactions Using CLIP-Seq.- Identification of Endogenous mRNA-Binding
Proteins in Yeast Using Crosslinking and PolyA Enrichment.- Ribo-Proteomics
Approach to Profile RNA-Protein and Protein-Protein Interaction Networks.- Detection
of Protein-Protein Interaction Within an RNA-Protein Complex Via
Unnatural-Amino-Acid-Mediated Photochemical Crosslinking.- Evolution of
Cell-Type-Specific RNA Aptamers Via Live Cell-Based SELEX.- mCarts: Genome-Wide
Prediction of Clustered Sequence Motifs as Binding Sites for RNA Binding
Proteins.- Design of RNA-Binding Proteins: Manipulate Alternative Splicing in
Human Cells with Artificial Splicing Factors.- Informational Suppression to
Probe RNA:RNA Interactions in the Context of Ribonucleoproteins: U1 and 5’ Splice-Site
Base-Pairing.- Analysis of Alternative Pre-RNA Splicing in the Mouse Retina
Using a Fluorescent Reporter.