Toll-Like Receptors -

Toll-Like Receptors

Practice and Methods

Claire McCoy (Herausgeber)

Buch | Softcover
455 Seiten
2018 | Softcover reprint of the original 2nd ed. 2016
Humana Press Inc. (Verlag)
978-1-4939-8023-9 (ISBN)
117,69 inkl. MwSt
This second edition volume expands on the first edition with new developments on Toll-Like Receptors (TLRs) controlling events such as cross-priming of associated pattern recognition receptors, post-transcriptional regulation, interaction with other cellular and biologic systems, and cancer progression. This book is divided into five sections: Part I outlines methods for TLR detection, interaction, and intracellular trafficking; Part II describes methods and assays to investigate how TLRs cross-prime other pattern recognition receptors, including intracellular DNA receptors and inflammasome formation; Part III highlights RNA regulation, detailing how TLRs can induce RNA transcripts and molecules such as lncRNAs and microRNAs; Part IV explores TLR detection and activation in systems such as epithelial barrier function, metabolism and the circadian clock, as well as cellular systems including T and B lymphocytes; and Part V describes models to delineate the role of TLRs in diseases such as dermatitis, arthritis, and gastric cancer. Written in the highly successful Methods in Molecular Biology series format, each chapter contains a summary, a list of required materials, step-by-step, readily reproducible laboratory protocols, useful notes to investigate TLRs in cell culture, systems and disease, and tips on troubleshooting and avoiding known pitfalls.



Practical and cutting-edge, Toll-Like Receptors: Methods and Protocols, Second Edition is a valuable resource to any immunologist, molecular or medical biologist working in a laboratory setting. It will add skill to both students and the more advanced molecular biologist who wishes to learn a new technique or move to a different area within their current repertoire of practical knowledge.

Toll-Like
Receptors: Ligands, Cell-Based Models, and Readouts for Receptor
Action.- Bioinformatic Analysis of Toll-Like Receptor Sequences and
Structures.- Toll-Like Receptor Interactions Measured by Microscopic and
Flow Cytometric FRET.- Using Confocal Microscopy to Investigate
Intracellular Trafficking of Toll-Like Receptors.- Assessing the
Inhibitory Activity of Oligonucleotides on TLR7 Sensing.- Methods for
Delivering DNA to Intracellular Receptors.- Detection of Interaction
between Toll-Like Receptors and other Transmembrane Proteins by
Co-Immunoprecipitation Assay.- Flow Cytometry-Based Bead-Binding Assay for
Measuring Receptor Ligand Specificity.- Measuring Monomer-to-Filament
Transition of MAVS as an In Vitro Activity Assay for RIG-I-Like Receptors.- Co-Transcriptomic
Analysis by RNA Sequencing to Simultaneously Measure Regulated Gene Expression
in Host and Bacterial Pathogen.- Simple Methods to Investigate MicroRNA
Induction in Response to Toll-Like Receptors.- Determining the Function of
Long Noncoding RNA in Innate Immunity.- Analysis of Post-Transcriptional
Gene Regulation of Nod-Like Receptors via the 3’UTR.- TLR Function in
Murine CD4+ T Lymphocytes and their Role in Inflammation.- Flow
Cytometry Analysis of B Cell Activation and Antibody Responses Induced by
Toll-Like Receptors.- Toll-Like Receptor-Dependent Immune Complex Activation of
B Cells and Dendritic Cells.- Analysis of TLR-Induced Metabolic Changes in
Dendritic Cells Using the Seahorse XFe96 Extracellular Flux
Analyzer.- Toll-Like Receptor Signaling and the Control of Intestinal
Barrier Function.- Understanding the Role of Cellular Molecular Clocks in
Controlling the Innate Immune Response.- Methods to Investigate the Role
of Toll-Like Receptors in Allergic Contact Dermatitis.- Allergens and
Activation of the Toll-Like Receptor Response.- Investigating the Role of
Toll-Like Receptorsin Models of Arthritis.- Delineating the Role of
Toll-Like Receptors in the Neuro-Inflammation Model EAE.- The Use of MiRNA
Antagonists in the Alleviation of Inflammatory Disorders.- Investigating
the Role of Toll-Like Receptors in Mouse Models of Gastric Cancer.

Erscheinungsdatum
Reihe/Serie Methods in Molecular Biology ; 1390
Zusatzinfo 29 Illustrations, color; 29 Illustrations, black and white; XIV, 455 p. 58 illus., 29 illus. in color.
Verlagsort Totowa, NJ
Sprache englisch
Maße 178 x 254 mm
Themenwelt Medizin / Pharmazie Medizinische Fachgebiete
Studium Querschnittsbereiche Infektiologie / Immunologie
Naturwissenschaften Biologie Biochemie
ISBN-10 1-4939-8023-8 / 1493980238
ISBN-13 978-1-4939-8023-9 / 9781493980239
Zustand Neuware
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