Retinal Degeneration
Humana Press Inc. (Verlag)
978-1-4939-8668-2 (ISBN)
Authoritative and cutting-edge, Retinal Degeneration: Methods and Protocols, Second Edition aims to ensure successful scientific work in the further study of this vital field.
Identification and Analysis of Genes Associated with Inherited Retinal Diseases.- eQTL Analysis in Retinal Research.- Testing for known Retinal Degeneration Mutants in Mouse Strains.- CRISPR/Cas9 Gene Editing In Vitro and in Retinal Cells In Vivo.- Monitoring Surface Reactions by Combined Western Blot-ELISA Analysis.- Generation of Functional Retinal Pigment Epithelium from Human Induced Pluripotent Stem Cells.- Advanced Analysis of Photoreceptor Outer Segment Phagocytosis by RPE Cells in Culture.- Porcine RPE/Choroidal Explant Cultures.- The Mouse Retinal Organoid Trisection Recipe: Efficient Generation of 3D Retinal Tissue from Mouse Embryonic Stem Cells.- Cell Death Analysis in Retinal Cultures.- Fate Mapping In Vivo to Distinguish Bona Fide Microglia Versus Recruited Monocyte-derived Macrophages in Retinal Disease.- Light Damage Models of Retinal Degeneration.- Induction and Readout of Oxygen-Iinduced Retinopathy.- Generation and Analysis of Xenopus laevis Models of Retinal Degeneration using CRISPR/Cas9.- Gene Knockdown in Zebrafish (Danio rerio) as a Tool to Model Photoreceptor Diseases.- Drosophila melanogaster - A Valuable Genetic Model Organism to Elucidate the Biology of Retinitis Pigmentosa.- Retinal Fundus Imaging in Mouse Models of Retinal Diseases.- Phenotyping of Mouse Models with OCT.- Cell-specific Markers for the Identification of Retinal Cells and Subcellular Organelles by Immunofluorescence Microscopy.- Immuno-TEM/STEM in Retinal Research.- Noninvasive Two-photon Microscopy Imaging of Mouse Retina and Retinal Pigment Epithelium.- Cell-based Therapy for Retinal Disease: The New Frontier.- In Vitro Evaluation of AAV Vectors for Retinal Gene Therapy.- Nanoparticles Targeting Retinal and Choroidal Capillaries In Vivo.- Optimized Subretinal Injection Technique for Gene Therapy Approaches.
Erscheinungsdatum | 02.11.2018 |
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Reihe/Serie | Methods in Molecular Biology ; 1834 |
Zusatzinfo | 56 Illustrations, color; 52 Illustrations, black and white; XIV, 417 p. 108 illus., 56 illus. in color. With online files/update. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Studium |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Schlagworte | Angiography • eQTL • Leukocytes • Mouse Models • Organoids |
ISBN-10 | 1-4939-8668-6 / 1493986686 |
ISBN-13 | 978-1-4939-8668-2 / 9781493986682 |
Zustand | Neuware |
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