Gradient HPLC for Practitioners

Stavros Kromidas, PhD, works as an independent consultant for analytical chemistry, based in Saarbrucken (Germany). For more than 20 years he has regularly held lectures and training courses on HPLC, and has authored numerous articles and several books on various aspects of chromatography. He is the founder of NOVIA GmbH, a provider of professional training and consulting in analytical chemistry, and served as its CEO until 2001.

Aspects of Gradient Optimization (Stavros Kromidas)
Instrumental Influences on the Quality and Performance of Gradient Methods and Their Transfer Between Different HPLC Devices (Frank Steiner)
Optimization of a Reversed-Phase Gradient Separation Using EXCEL (Hans-Joachim Kuss)
Gradient Elution of Ionic Compounds (Joachim Weiss)
The Gradient in Biochromatography (Oliver Genz)
Specifications of Gradients in Hydrophilic Interaction Liquid Chromatography (HILIC) (Thomas Letzel)
Specifications of Gradients in Supercritical Fluid Chromatography (SFC) (Stefan Bieber, Thomas Letzel)
Aspects of Gradient Elution in LC-MS Analysis (Markus M. Martin)
Additional Tools for Method Development: Flow and Temperature Gradients (Egidijus Machtejevas)