Fluorescent Tools for Imaging Oxidative Stress in Biology (eBook)

(Autor)

eBook Download: PDF
2018 | 1st ed. 2018
XLV, 230 Seiten
Springer International Publishing (Verlag)
978-3-319-73405-7 (ISBN)

Lese- und Medienproben

Fluorescent Tools for Imaging Oxidative Stress in Biology - Amandeep Kaur
Systemvoraussetzungen
96,29 inkl. MwSt
  • Download sofort lieferbar
  • Zahlungsarten anzeigen

This thesis advances the long-standing challenge of measuring oxidative stress and deciphering its underlying mechanisms, and also outlines the advantages and limitations of existing design strategies. It presents a range of approaches for the chemical synthesis of fluorescent probes that detect reversible changes in cellular oxidative stress. The ability to visualise cellular processes in real-time is crucial to understanding disease development and streamline treatment, and this can be achieved using fluorescent tools that can sense reversible disturbances in cellular environments during pathogenesis. The perturbations in cellular redox state are of particular current interest in medical research, since oxidative stress is implicated in the pathogenesis of a number of diseases.

The book investigates different strategies used to achieve ratiometric fluorescence output of the reversible redox probes, which nullify concentration effects associated with intensity-based probes. It also describes suitable approaches to target these probes to specific cellular organelles, thereby enabling medical researchers to visualise sub-cellular oxidative stress levels, and addressing the typically poor uptake of chemical tools into biological studies.

In total it reports on four new probes that are now being used by over twenty research groups around the globe, and two of which have been commercialised. The final chapters of this thesis demonstrate successful applications of the sensors in a variety of biological systems ranging from prokaryotes to mammalian cells and whole organisms. The results described clearly indicate the immense value of collaborative, cross-disciplinary research.



Amandeep Kaur completed her master's studies in chemistry, with a University medal from VIT University, India in 2012. She then took up a research internship at ENSCCF, France, where she worked on the development of transition metal-doped calcium phosphate ceramics. Amandeep completed her Ph.D. in 2016 at the University of Sydney with Dr Elizabeth New, during which she was awarded a World Scholars Scholarship. Her Ph.D. research involved the development of reversible fluorescent redox sensors for understanding the role of oxidative stress in biological systems. She is currently working as a postdoctoral research fellow at the EMBL Australia Node in Single Molecule Science, University of New South Wales, Australia.

Amandeep Kaur completed her master’s studies in chemistry, with a University medal from VIT University, India in 2012. She then took up a research internship at ENSCCF, France, where she worked on the development of transition metal-doped calcium phosphate ceramics. Amandeep completed her Ph.D. in 2016 at the University of Sydney with Dr Elizabeth New, during which she was awarded a World Scholars Scholarship. Her Ph.D. research involved the development of reversible fluorescent redox sensors for understanding the role of oxidative stress in biological systems. She is currently working as a postdoctoral research fellow at the EMBL Australia Node in Single Molecule Science, University of New South Wales, Australia.

Introduction.- Flavin based redox probes.- FRET based ratiometric redox probes.- Mitochondrially-targeted ratiometric redox probes.- Nicotinamide based ratiometric probes.- In cellulo studies.- Ex vivo studies.- Non-mammalian systems.- Conclusions.- Experimental Methods. 

Erscheint lt. Verlag 2.3.2018
Reihe/Serie Springer Theses
Springer Theses
Zusatzinfo XLV, 230 p. 161 illus., 100 illus. in color.
Verlagsort Cham
Sprache englisch
Themenwelt Naturwissenschaften Biologie
Naturwissenschaften Chemie
Schlagworte biological imaging • cellular redox state • Embryonic Stem Cells • Ex Vivo Studies • Flavin-based Redox Probes • FRET-based Redox Probes • Nicotinamide-based Redox Probes • Oxidative stress • Pancreatic Cancer Cells • Ratiometric Fluorescent Probes
ISBN-10 3-319-73405-9 / 3319734059
ISBN-13 978-3-319-73405-7 / 9783319734057
Haben Sie eine Frage zum Produkt?
PDFPDF (Wasserzeichen)
Größe: 11,3 MB

DRM: Digitales Wasserzeichen
Dieses eBook enthält ein digitales Wasser­zeichen und ist damit für Sie persona­lisiert. Bei einer missbräuch­lichen Weiter­gabe des eBooks an Dritte ist eine Rück­ver­folgung an die Quelle möglich.

Dateiformat: PDF (Portable Document Format)
Mit einem festen Seiten­layout eignet sich die PDF besonders für Fach­bücher mit Spalten, Tabellen und Abbild­ungen. Eine PDF kann auf fast allen Geräten ange­zeigt werden, ist aber für kleine Displays (Smart­phone, eReader) nur einge­schränkt geeignet.

Systemvoraussetzungen:
PC/Mac: Mit einem PC oder Mac können Sie dieses eBook lesen. Sie benötigen dafür einen PDF-Viewer - z.B. den Adobe Reader oder Adobe Digital Editions.
eReader: Dieses eBook kann mit (fast) allen eBook-Readern gelesen werden. Mit dem amazon-Kindle ist es aber nicht kompatibel.
Smartphone/Tablet: Egal ob Apple oder Android, dieses eBook können Sie lesen. Sie benötigen dafür einen PDF-Viewer - z.B. die kostenlose Adobe Digital Editions-App.

Buying eBooks from abroad
For tax law reasons we can sell eBooks just within Germany and Switzerland. Regrettably we cannot fulfill eBook-orders from other countries.

Mehr entdecken
aus dem Bereich