Planarian Regeneration

Jochen Rink, PhD Group Leader Max Planck Institute of Molecular Cell Biology and Genetics Pfotenhauerstrasse 108, 01307 Dresden, Germany Tel: +49-(0)351-210-2435 Email: rink@mpi-cbg.de 

Planarian Diversity and Phylogeny.- Stem Cells, Patterning, and Regeneration in Planarians-Self-Organization at the Organismal Scale.- The Ecology of Freshwater Planarians.- Planarians and the History of Animal Regeneration: Paradigm Shifts and Key Concepts in Biology.- Culturing Planarians in the Laboratory.- Total RNA Isolation from Planarian Tissues.- Small- and Large-Scale High Molecular Weight Genomic DNA Extraction from Planarians.- Planarian High Molecular Weight DNA Isolation by Spooling.- Basic Histological Techniques for Planarians.- Fixation, Processing, and Immunofluorescent Labeling of Whole Mount Planarians.- Immunohistochemistry on Paraffin-Embedded Planarian Tissue Sections.- Whole-Mount In Situ Hybridization of Planarians.- RNA In Situ Hybridization on Planarian Paraffin Sections.- General Principles of Planarian Embryogenesis and Its Analysis by In Situ Hybridization and Immunohistochemistry Methods.- Whole-Mount BrdU Staining with Fluorescence In Situ Hybridization in Planarians.- Detection of Apoptotic Cells in Planarians by Whole-Mount TUNEL.- Systemic RNA Interference in Planarians by Feeding of dsRNA Containing Bacteria.- RNA Interference in Planarians: Feeding and Injection of Synthetic dsRNA.- A Subtractive FACS Method for Isolation of Planarian Stem Cells and Neural Cells.- Clonal Analysis of Planarian Stem Cells by Subtotal Irradiation and Single-Cell Transplantation.- Tissue Transplantations in Planarians.- Live Imaging Planaria.- Processing Schmidtea mediterranea for Transmission Electron Microscopy: Classical and Microwave Techniques.- Tissue Extracts for Quantitative Mass Spectrometry of Planarian Proteins Using SILAC.- Quantification of Neurotransmitters from Intact and Regenerating Planarians Using UHPLC-MS/SRM Method.