Membrane Potential Imaging in the Nervous System and Heart

Marco Canepari (b. Milan 1970) is first class INSERM researcher (CR1) working at the Laboratoire Interdisciplinaire de Physique in Grenoble. He graduated in physics at the University of Genoa and received his PhD in biophysics from the International School for Advanced Studies in Trieste. He worked at the National Institute for Medical Research in London, at Yale University and at the University of Basel. Marco is expert on several optical techniques applied to neurophysiology. Marco and Dejan collaborated for a number of years using voltage-imaging and calcium imaging approaches to study mechanisms underlying synaptic plasticity. Olivier Bernus (b. Tournai 1976) is Professor and Scientific Director at the Electrophysiology and Heart Modeling Institute LIRYC at the University of Bordeaux, France. He graduated in Mathematical Physics at the University of Ghent, Belgium and obtained his PhD in Physics from the same university. He subsequently worked as a postdoctoral fellow at SUNY Upstate Medical University, USA, and as a tenure track research fellow at the University of Leeds, UK. Olivier is an expert in cardiac optical imaging and is currently working on the development of novel techniques for three-dimensional depth-resolved optical imaging of cardiac electrical activity. Dejan Zecevic (b. Belgrade 1948) is Senior Research Scientist at the Department of Cellular and Molecular Physiology, Yale University School of Medicine. He received the PhD in Biophysics from The University of Belgrade, Serbia and was trained in the laboratory of Dr Lawrence Cohen who initiated the field of voltage-sensitive dye recording. Dejan is the pioneer of intracellular voltage-sensitive dye imaging technique, a unique and a cutting edge technology for monitoring the membrane potential signaling in axons, dendrites and dendritic spines of individual nerve cells.

Part 1 Principles of Membrane Potential-Imaging.- Historical Overview and General Methods of Membrane Potential-Imaging.- Design and Use of Organic Voltage-Sensitive Dyes.- Part 2 Membrane Potential Signals with Single Cell Resolution.- Imaging Sub millisecond Membrane Potential Changes from Individual Regions of Single Axons, Dendrites and Spines.- Combining Membrane Potential Imaging with Other Optical Techniques.- Monitoring Spiking Activity of Many Individual Neurons in Invertebrate Ganglia.- Part 3 Monitoring Activity of Networks and Large Neuronal Populations.- Monitoring Integrated Activity of Individual Neurons Using FRET-Based Voltage-Sensitive Dyes.- Monitoring Population Membrane Potential Signals from Neocortex.- Voltage Imaging in the Study of Hippocampal Circuit Function and Plasticity.- Monitoring Population Membrane Potential Signals During Development of The Vertebrate Nervous System.- Imaging the Dynamics Of Mammalian Neocortical Population Activity In-Vivo .- Imaging the Dynamics of Neocortical Population Activity in Behaving and Freely Moving Mammals.- Part 4 Monitoring Membrane Potential in the Heart.- History of Cardiac Optical Imaging.- Imaging of Ventricular Fibrillation and Defibrillation: The Virtual Electrode Hypothesis.- Optical Mapping of Ventricular Fibrillation Dynamics.- Bio photonics Modelling of Cardiac Optical Imaging.- Towards Depth-Resolved Optical Imaging of Cardiac Electrical Activity.- Part 5 New Approaches - Potentials and Limitations.- Two-Photon Excitation of Fluorescent Voltage-Sensitive Dyes: Monitoring Membrane Potential in the Infrared.- Random-Access Multi photon Microscopy for Fast Three-Dimensional Imaging.- High Spatial Resolution Microscopy Using Holographic Illumination.- Second Harmonic Imaging of Membrane Potential.- Genetically Encoded Protein Sensors of Membrane Potential.