Protocols for Macroalgae Research

Dr. Bénédicte Charrier is a senior scientist from CNRS working at Station Biologique Roscoff, France. She is a specialist of macroalgae morphogenesis and development, with an expertise on the filmentous alga Ectocarpus siliculosus. She is currently the Chair of the European network "Phycomorph", supported by the Cooperation in Science & Technology (COST) Association (2015-2019), which aims to coordinate research in growth, reproduction, and morphogenesis of macroalgae in Europe and in association with Asian and North American laboratories. Dr. Thomas Wichard is a research group leader at the Institute for　Inorganic and Analytical Chemistry of the Friedrich Schiller　University Jena. The main focus of his research group is to elucidate　the mutualistic interactions between bacteria and the marine macroalga　Ulva ("cross-kingdom-cross-talk"). The group applies various　methodologies in analytical chemistry, chemical ecology and molecular　biology to understand the basis of eco-physiological processes. Dr. CRK Reddy is a Chief Scientist in Seaweed Biology and Biotechnology Cultivation at CSIR-Central Salt and Marine Chemicals Research Institute in Bhavnagar, India. He is an expert in marine macroalgae, making significant contributions to macroalgae tissue culture and protoplast techniques including genetic improvement of seaweeds, seaweed biomass for chemicals and biofuels, seaweed biodiversity, and seaweed biology and cultivation.

CONTENTS

Section I: Cultivating and preserving seaweeds

Chapter 1 Seaweed in high-energy environments: Protocol

to move Saccharina cultivation offshore

Chapter 2 Cultivation protocol for Saccharina latissima

Chapter 3 Derivation of clonal stock cultures and hybridization

of kelps: A tool for strain preservation and breeding

programs

Chapter 4 Cryopreservation of macroalgae

Chapter 5 Unraveling seaweeds bacteriomes: From field site

to computer screen

Chapter 6 Heavy metal ecotoxicity on the early life history

stages of macroalgae

Chapter 7 A simple protocol for a rapid and consistent

production of a large number of viable protoplasts

from the Ulvophycean species

Chapter 8 Purification of sporulation and swarming inhibitors

from Ulva: Application in algal life-cycle controlling

Chapter 9 Preparation of axenic cultures in Ulva (Chlorophyta)

Section II: Chemical composition

Chapter 10 Biochar production from seaweeds

Chapter 11 Identification and quantification of laminarins

in brown algae

of macroalgae

Chapter 12 Determination of carbohydrate composition

Chapter 13 Quantification of proteins in seaweeds

Chapter 14 Comprehensive phytohormone quantification

in the red alga Pyropia yezoensis by liquid

chromatography–mass spectrometry

Chapter 15 Total phenolic content and antioxidant capacity

analysis of seaweed biomass

Chapter 16 Extraction of phycocyanin and phycoerythrin pigments

Chapter 17 Quantification and localization of reactive oxygen

species in marine macrophytes

Chapter 18 Metabolomics of intra- and extracellular metabolites

from micro- and macroalgae using GC–MS

and LC–MS

Chapter 19 Preparative extraction of exometabolites

from seaweed surfaces

Florian Weinberger

Chapter 20 Disruption-free solid-phase extraction of surface

metabolites from macroalgae

Emilio Cirri and Georg Pohnert

Section III: Cellular and molecular characterization

Chapter 21 The immunodetection and in situ imaging of

cell-wall polysaccharides in brown algae

Chapter 22 Atomic force microscopy based analysis of cell-wall

elasticity in macroalgae

Chapter 23 Dynamic and microscale mapping of cell growth:

Case of Ectocarpus filament cells

Chapter 24 Actin fluorescent staining in the filamentous brown

alga Ectocarpus siliculosus

Chapter 25 Cryofixation of brown algae for transmission

electron microscopy

Chapter 26 Probing the subcellular topography of

seaweeds: Transmission electron microscopy,

immunocytochemistry, and correlative light

microscopy

Chapter 27 Coralline algae preparation for scanning electron

microscopy and optical microscopy

Chapter 28 Extraction of high quality RNA from brown algae

for transcriptomic analysis

Chapter 29 Induction of sexual reproduction in Spirogyra

cultures for laser capture microdissection of gametes

and zygotes

Chapter 30 Cloning and expression strategies for the

postgenomic analysis of brown algae

Chapter 31 Polyethylene glycol-mediated transformation in

the green macroalga Ulva mutabilis (Chlorophyta):

A forward genetics approach

Index