Natural Killer Cell Protocols

Purification of Human NK Cell Developmental Intermediates from Lymph Nodes and Tonsils.- In Vitro Development of Human Killer–Immunoglobulin Receptor-Positive NK Cells.- Subset Analysis of Human and Mouse Mature NK Cells.- Assessing Licensing of NK Cells.- Use of Stem Cell Radiation Chimeras to Analyze How Domains of Specific Proteins Impact on Murine NK Cell Development In Vivo.- Use of Transfected Drosophila S2 Cells to Study NK Cell Activation.- Natural Killer Cell Conjugate Assay Using Two-Color Flow Cytometry.- Studying NK Cell/Dendritic Cell Interactions.- Analysis of the NK Cell Immunological Synapse.- Measuring Intracellular Calcium Signaling in Murine NK Cells by Flow Cytometry.- Intracellular Staining for Analysis of the Expression and Phosphorylation of Signal Transducers and Activators of Transcription (STATs) in NK Cells.- A Model System for Studying NK Cell Receptor Signaling.- Expression of cDNAs in Human Natural Killer Cell Lines by Retroviral Transduction.- Lentiviral Gene Transduction in Human and Mouse NK Cell Lines.- of shRNAs into Human NK-Like Cell Lines with Retrovirus.- of shRNAs into Primary NK Cells with Lentivirus.- Methods to Identify and Characterize Different NK Cell Receptors and Their Ligands.- Generating NK Cell Receptor-Fc Chimera Proteins from 293T Cells and Considerations of Appropriate Glycosylation.- Identification of NK Cell Receptor Ligands Using a Signaling Reporter System.- Determining Ligand Specificity of Ly49 Receptors.- Probing the Interactions of NK Cell Receptors with Ligand Expressed in trans and cis.- A Simple Method to Measure NK Cell Cytotoxicity In Vivo.- Functional Analysis of Human NK Cells by Flow Cytometry.- Analysis of the KIR Repertoire in Human NK Cells by Flow Cytometry.- KIR Genotyping by MultiplexPCR-SSP.- Identification and Analysis of Novel Transcripts and Promoters in the Human Killer Cell Immunoglobulin-like Receptor (KIR?) Genes.- Use of Inbred Mouse Strains to Map Recognition Receptors of MCMV Infected Cells in the NK Cell Gene Locus.- Studying NK Cell Responses to Ectromelia Virus Infections in Mice.- Activation of Human NK Cells by Malaria-Infected Red Blood Cells.- Natural Killer Cells in Human Pregnancy.- Analysis of Uterine Natural Killer Cells in Mice.- Isolation of NK Cells and NK-Like Cells from the Intestinal Lamina Propria.