Phospho-Proteomics
Springer-Verlag New York Inc.
978-1-4939-5001-0 (ISBN)
Authoritative and practical, Phosphoproteomics Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
Thiol-ene Enabled Detection of Thiophosphorylation as a Labeling Strategy for Phosphoproteins.- Phosphopeptide Detection with Biotin-Labeled Phos-tag.- Phosphopeptide Enrichment by Covalent Chromatography after Solid Phase Derivatization of Protein Digests on Reversed Phase Supports.- Peptide Labeling Using Isobaric Tagging Reagents for Quantitative Phosphoproteomics.- Identification of Direct Kinase Substrates Using Analogue-Sensitive Alleles.- Quantitative Analysis of Tissue Samples by Combining iTRAQ Isobaric Labeling with Selected/Multiple Reaction Monitoring (SRM/MRM).- Enrichment Strategies in Phosphoproteomics.- Phosphopeptide Enrichment by Immobilized Metal Affinity Chromatography.- The use of Titanium Dioxide for Selective Enrichment of Phosphorylated Peptides.- Sequential Elution from IMAC (SIMAC) – An Efficient Method for Enrichment and Separation of Mono- and Multi-Phosphorylated Peptides.- Improving the Phosphoproteome Coverage for Limited Sample Amounts Using TiO2-SIMAC-HILIC (TiSH) Phosphopeptide Enrichment and Fractionation.- Offline High pH Reversed-Phase Peptide Fractionation for Deep Phosphoproteome Coverage.- Phosphopeptide Enrichment Using Various Magnetic Nanocomposites – An Overview.- Two Dimensional Gel Electrophoresis-Based Plant Phosphoproteomics.- Variable Digestion Strategies for Phosphoproteomics Analysis.- On-line LC-FAIMS-MS/MS for the Analysis of Phosphorylation in Proteins.- Simple and Reproducible Sample Preparation for Single Shot Phosphoproteomics With High Sensitivity.- Identification of Direct Kinase Substrates via Kinase Assay Linked Phosphoproteomics.- Phosphoprotein Detection by High-Throughput Flow Cytometry.- Resources for Assignment of Phosphorylation Sites on Peptides and Proteins.- From Phosphosites to Kinases.- Search Databases and Statistics – Pitfalls and Best Practices in Phosphoproteomics.- Systems Analysis for Interpretation of Phosphoproteomics Data.
Erscheinungsdatum | 19.08.2017 |
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Reihe/Serie | Methods in Molecular Biology ; 1355 |
Zusatzinfo | XIII, 364 p. |
Verlagsort | New York |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Allgemeines / Lexika |
Naturwissenschaften ► Biologie ► Biochemie | |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Schlagworte | kinase-substrate relationships • phospho flow cytometry • phosphopeptides • phosphoproteins • phosphorylated proteins • quantitative phosphoproteomics • Ser residues • Thr residues • tissue samples • Tyr residues |
ISBN-10 | 1-4939-5001-0 / 1493950010 |
ISBN-13 | 978-1-4939-5001-0 / 9781493950010 |
Zustand | Neuware |
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