TALENs

Methods and Protocols

Ralf Kühn, Wolfgang Wurst, Benedikt Wefers (Herausgeber)

Buch | Softcover

274 Seiten

2016 | Softcover reprint of the original 1st ed. 2016
Humana Press Inc. (Verlag)
978-1-4939-4905-2 (ISBN)

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This volume provides a comprehensive collection of protocols on new technology across various model organisms. Chapters describe species-specific methods to generate new mutants the content is completed by chapters on natural TAL effectors, TAL element DNA binding principles, TALEN target site prediction, and methods for the efficient construction of TALEN coding regions. While addition chapter focus on the application of TALEN as sequence-specific nucleases and TAL based gene activators or inhibitors and the visualization of chromatin dynamics in live cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, TALENs: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Xanthomonas and the TAL effectors: Nature's Molecular Biologist.- TAL Effector DNA-binding Principles and Specificity.- The Development of TALE Nucleases for Biotechnology.- Online Tools for TALEN Design.- Assembly of Customized TAL Effectors through Advanced ULtiMATE System.- Engineering Customized TALENs Using the Platinum Gate TALEN Kit.- Design, Assembly, and Characterization of TALE-Based Transcriptional Activators and Repressors.- A New Approach to Dissect Nuclear Organization; TALE-mediated Genome Visualization (TGV).- TALEN Induced Translocations in Human Cells.- Mutagenesis in Newts; Protocol for Iberian Ribbed Newts.- Targeted Mutagenesis in Bombyx mori Using TALENs.- GeneKnockout by Targeted Mutagenesis in a Hemimetabolous Insect, The TwoSpotted Cricket Gryllus bimaculatus, using TALENs.- Methods for TALEN Evaluation, use, and Mutation Detection in the Mosquito Aedes aegypti.- Methods for TALEN-mediated Genomic Manipulations in Drosophila.- Targeted Mutagenesis in Zebrafish by TALENs.- Mutagenesis in Xenopus and Zebrafish using TALENs.- Genome Editing in Mice using TALE Nucleases.- Genome Editing in Rats using TALE Nucleases.- Designer Nuclease-mediated Generation of Knock out THP1 Cells.

Erscheinungsdatum	19.08.2017
Reihe/Serie	Methods in Molecular Biology ; 1338
Zusatzinfo	50 Illustrations, color; 15 Illustrations, black and white; XII, 274 p. 65 illus., 50 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete
	Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Naturwissenschaften ► Biologie ► Zoologie
Schlagworte	chromatin dynamics in live cells • DNA sequence recognition • natural TAL effectors • nuclease technology • sequence-specific nucleases • short RNAs • TAL based gene activators • TAL element DNA binding principles • TALEN coding regions • TALEN target site prediction
ISBN-10	1-4939-4905-5 / 1493949055
ISBN-13	978-1-4939-4905-2 / 9781493949052
Zustand	Neuware