The BAM Complex

The β-barrel Assembly Machinery Comple.- Yeast Mitochondria as a Model System to Study the Biogenesis of Bacterial β-barrel Proteins.- Experimental Methods for Studying the BAM Complex in Neisseria meningitides.- Heat Modifiability of Outer Membrane Proteins from Gram-Negative Bacteria.- The Role of a Destabilized Membrane for OMP Insertion.- Treponema pallidum in Gel Microdroplets: A Method for Topological Analysis of BamA (TP0326) and Localization of Rare Outer Membrane Proteins.- Analyzing the Role of Periplasmic Folding Factors in the Biogenesis of OMPs and Members of the Type V Secretion System.- An In Vitro Assay for Substrate Translocation by FhaC in Liposomes.- Measuring Cell-cell Binding Using Flow-Cytometry.- Methods to Characterize Folding and Function of BamA Crosslink Mutants.- Small Angle X-ray Scattering (SAXS) Characterization of the POTRA Domains of BamA.- Assessing the Outer Membrane Insertion and Folding of Multimeric Transmembrane β-barrel Proteins.- The Expression, Purification, and Structure Determination of BamA from E. coli.- Expression and Purification of the Individual Bam Components BamB-E.- Structure Determination of the BAM Complex Accessory Lipoproteins BamB-E.- An In Vitro Assay for Outer Membrane Protein Assembly by the BAM complex.- Identification of BamC on the Surface of E. coli.- Construction and Characterization of an E. coli bamD Depletion Strain.- Expression, Purification and Screening of BamE, a Component of the BAM Complex, for Structural Characterization.- Purification and Bicelle Crystallization for Structure Determination of the E. coli Outer Membrane Protein TamA.- Strategies for the Analysis of Bam Recognition Motifs in Outer Membrane Proteins.- Summary and Future Directions.