Practical Manual of In Vitro Fertilization

Journey of Human Gametes In Vitro: 1978 to 2010.- Building the Laboratory.- Air Quality Management.- Organizational Aspects of the Laboratory in a Tertiary Care ART Center.- Quality Control Management.- Daily, Weekly, and Regular Preparations for the IVF Laboratory.- Risk and Safety in the IVF Clinic.- Essential Instruments and Disposable Supplies for an IVF Laboratory.- Co2 and Low-O2 Incubators.- IVF Workstations.- Culture Media in IVF: Decisions for the Laboratory.- Oocyte Denuding.- Assessment of Oocyte Quality.- Polarization Microscopy.- Cumulus Cell Gene Expression in Assessment of Oocyte Quality.- Short Culture: Day 1/Day 2/Day 3 Embryo Culture.- Extended Culture in IVF.- In Vitro Maturation of Human Oocytes.- In Vivo Embryo Culture Device.- Microfluidics for Gamete Manipulation and Embryo Culture.- Sperm Assessment: Traditional Approaches and Their Indicative Value.- Sperm Assessment: Novel Approaches and Their Indicative Value.- Sperm Processing for IVF.- PESA/TESA/TESE Sperm Processing.- Processing Sperm Samples in HIV-Positive Patients.- Intracytoplasmic Morphologically Selected Sperm Injection.- Sperm Testing and ICSI Selection by Hyaluronic Acid Binding: The Hyaluronic Acid-Coated Glass Slide and Petri Dish in the Andrology and IVF Laboratories.-  Electrophoretic Sperm Separation.- Magnetic Activated Cell Sorting of Human Spermatozoa.- Poloscope-Based Sperm Selection.- Intrauterine Insemination.- Conventional IVF Insemination.- Intracytoplasmic Sperm Injection.- Mechanism of Human Oocyte Activation During ICSI and Methodology for Overcoming Low or Failed Fertilization.- Hydraulic Manipulators for ICSI.- Research Instruments Micromanipulators.- Eppendorf Micromanipulator: Setup and Operation of Electronic Micromanipulators.- The Leica-IMSI System.- Automated Robotic Intracytoplasmic Sperm Injection.- Oocyte Treatment and Preparation for Microinjection.- Livestock Production Via Micromanipulation.- Pronuclear Scoring in Human In Vitro Fertilization.- Cumulative Morphological Assessment of Embryo Quality.- Metabolomics: The ViaMetrics-E™ Procedure for Assessing Embryo Viability.- Oxygen Consumption as an Indicator of Oocyte and Embryo Viability.- Gene Expression Changes During Human Early Embryo Development: New Applications for Embryo Selection.- Amino Acid Turnover as a Biomarker of Embryo Viability.- Real-time Embryo Monitoring Devices for Embryo Selection.- Assisted Hatching in IVF.- Polar Body Biopsy.- Cleavage Stage Embryo Biopsy.- Embryo Biopsy for PGD: Current Perspective.- Microarrays and CGH for PGD of Chromosome Abnormalities and Gene Defects.- Sperm Cryopreservation.- Slow Freezing of Oocytes.- Vitrification: Research in Animal Models.- Oocyte Vitrification.- Slow Freezing of Embryos.- Vitrification of Embryos.- Directional Freezing of Reproduction Cells, Tissues, and Organs.- Ovarian Tissue Cryopreservation.- Assessment of Uterine Receptivity.- Single Embryo Transfer.- Ultrasound-Guided Embryo Transfer (Abdominal/Vaginal): An Evidence-Based Evaluation.- Cumulus-Aided Embryo Transfer.- Data Management in the ART Laboratory: Requirements and Solutions.- Regulation, Licensing, and Accreditation of the ART Laboratory.- Legislation in the United Kingdom.- Regulation, Licensing, and Accreditation of the ART Laboratory in Europe.- Regulation, Licensing, and Accreditation of ART Laboratories in India.- Troubleshooting in the Clinical Embryology Laboratory: The Art of Problem-Solving in ART.- The Role of Mitochondria in the Establishment of Developmental Competence in Early Human Development.- Nuclear and Cytoplasmic Transfer: Human Applications and Concerns.- Cytoskeletal Architecture of Human Oocytes with Focus on Centrosomes and Their Significant Role in Fertilization.- Molecular Mining of Follicular Fluid for Reliable Biomarkers of Human Oocyte and Embryo Developmental Competence.