Ribosome Display and Related Technologies
Humana Press Inc. (Verlag)
978-1-4939-5872-6 (ISBN)
Invaluable and easy to use, Ribosome Display and Related Technologies: Methods and Protocols will be of great benefit to those with general molecular biology or protein engineering experience who wish to select peptides or proteins by display, those with phage display experience who would benefit from the application of ribosome display, as well as those with some ribosome display experience who would like to expand the range of applications to which they are applying the technology.
Ribosome Display: A Perspective.- Preparation and Testing of E.coli S30 In Vitro Transcription Translation Extracts.- Eukaryotic Ribosome Display Selection Using Rabbit Reticulocyte Lysate.- Stabilized Ribosome Display for In Vitro Selection.- Eukaryotic Ribosome Display with In Situ DNA Recovery.- mRNA Display Using Covalent Coupling of mRNA to Translated Proteins.- SNAP Display: In Vitro Protein Evolution in Microdroplets.- cDNA Display: Rapid Stabilization of mRNA Display.- Optimization of Antibody Affinity by Ribosome Display Using Error-Prone or Site-Directed Mutagenesis.- Affinity Maturation of Phage Display Antibody Populations using Ribosome Display.- Evolution of Protein Stability Using Ribosome Display.- Selection of Lead Antibodies from Naive Ribosome Display Antibody Libraries.- Evolution of Disulfide-Rich Peptide Aptamers Using cDNA Display.- Peptide Screening Using PURE Ribosome Display.- Rapid Selection of High Affinity Binders Using Ribosome Display.- mRNA Display-Based Selections Using Synthetic Peptide and Natural Protein Libraries.- Identification of Candidate Vaccine Genes Using Ribosome Display.- Ribosome Display for the Selection of Sac7d Scaffolds.- Charging of tRNAs Using Ribozymes and Selection of Cyclic Peptides Containing Thioethers.- Update on Pure Translation Display with Unnatural Amino Acid Incorporation.- In Vitro Selection of Unnatural Cyclic Peptide Libraries Via mRNA Display.- Optimization of CAT-354, a Therapeutic Antibody Directed Against Interleukin-13, Using Ribosome Display.-Affinity Maturation and Functional Dissection of a Humanized Anti-RAGE Monoclonal Antibody by Ribosome Display.
Erscheinungsdatum | 19.08.2017 |
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Reihe/Serie | Methods in Molecular Biology ; 805 |
Zusatzinfo | XIII, 424 p. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Allgemeines / Lexika |
Naturwissenschaften ► Biologie ► Biochemie | |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
ISBN-10 | 1-4939-5872-0 / 1493958720 |
ISBN-13 | 978-1-4939-5872-6 / 9781493958726 |
Zustand | Neuware |
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