Human Embryonic Stem Cells Handbook

Establishment of New Lines of Human Embryonic Stem Cells: Evolution of the Methodology.- Successful Scale-Up and Quality Assessments of Human Embryonic Stem Cells for Cell Therapy: Challenges and Overview.- Human Embryonic Stem Cells Derived in Xeno-Free Conditions.- Procedures for Derivation and Characterization of Human Embryonic Stem Cells from Odense, Denmark.- Principles for Derivation of Human Embryonic Stem Cells.- Derivation and Maintenance of Undifferentiated Human Embryonic Stem Cells.- Establishment of hESC Lines from the Inner Cell Mass of Blastocyst-Stage Embryos and Single Blastomeres of 4-Cell Stage Embryos.- Analysis of LINE-1 Expression in Human Pluripotent Cells.- Characterization and Gene Expression Profiling of Five Human Embryonic Stem Cell Lines Derived in Taiwan.- Derivation of Human Embryonic Stem Cell Lines from Poor Quality Embryos.- Derivation, Expansion, and Characterization of Human Embryonic Stem Cell Lines from Aneuploid Embryos.- Mutated Human Embryonic Stem Cells for the Study of Human Genetic Disorders.- Single-Cell Enzymatic Dissociation of hESC Lines OxF1--OxF4 and Culture in Feeder-Free Conditions.- Protocol for Expansion of Undifferentiated Human Embryonic and Pluripotent Stem Cells in Suspension.- Suspension Bioreactor Expansion of Undifferentiated Human Embryonic Stem Cells.- Derivation, Propagation, and Characterization of Neuroprogenitors from Pluripotent Stem Cells (hESCs and hiPSCs).- Comparison of Neural Differentiation Potential of Human Pluripotent Stem Cell Lines Using a Quantitative Neural Differentiation Protocol.- Array-Comparative Genomic Hybridization Characterization of Human Pluripotent Stem Cells.- Chromatin Immunoprecipitation-Based Analysis of Gene Regulatory Networks Operative in Human Embryonic Stem Cells.- Analysis of the Methylome of Human Embryonic Stem Cells Employing Methylated DNA Immunoprecipitation Coupled to Next Generation Sequencing (MeDIP-seq).- Stable Isotope Labelling with Amino Acids in Cell Culture for Human Embryonic Stem Cell Proteomic Analysis.