Mammalian Cell Viability
Humana Press Inc. (Verlag)
978-1-4939-6153-5 (ISBN)
Practical and adaptable, Mammalian Cell Viability: Methods and Protocols serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incredibly important and influential field.
Cell Viability Assays: Introduction.- Cell Viability Analysis Using Trypan Blue: Manual and Automated Methods.- Estimation of Cell Number Based on Metabolic Activity: The MTT Reduction Assay.- WST8 Analysis of Cell Viability During Osteogenesis of Human Mesenchymal Stem Cells.- Assessment of Cell Proliferation with Resazurin-Based Fluorescent Dye.- The xCELLigence System for Real-Time and Label-Free Monitoring of Cell Viability.- Analysis of Tumor and Endothelial Cell Viability and Survival Using Sulforhodamine B (SRB) and Clonogenic Assays.- Annexin V / 7-AAD Staining in Keratinocytes.- Measurement of Caspase Activity: From Cell Populations to Individual Cells.- Rapid Quantification of Cell Viability and Apoptosis in B-Cell Lymphoma Cultures Using Cyanine SYTO Probes.- Multiplexing Cell Viability Assays.- Cytotoxicity Testing: Measuring Viable Cells, Dead Cells, and Detecting Mechanism of Cell Death.- Fluorescein Diacetate for Determination of Cell Viability in 3D Fibroblast-Collagen-GAG Constructs.- Confocal Imaging Protocols for Live/Dead Staining in 3-Dimensional Carriers.- Viability Assessment of Osteocytes Using Histological Lactate Dehydrogenase Activity Staining on Human Cancellous Bone Sections.- Measuring Glutamate Receptor Activation-Induced Apoptotic Cell Death in Ischemic Rat Retina Using the TUNEL Assay.- Exploiting the Liberation of Zn2+ to Measure Cell Viability.- Non-Invasive Bioluminescent Quantification of Viable Stem Cells in Engineered Constructs.- Raman Micro-Spectroscopy as a Non-Invasive Cell Viability Test.- Closed Ampoule Isothermal Microcalorimetry (IMC) for Continuous Real-Time Detection and Evaluation of Cultured Mammalian Cell Activity and Responses.-Digital Image Processing of Live/Dead Staining.
Erscheinungsdatum | 19.08.2017 |
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Reihe/Serie | Methods in Molecular Biology ; 740 |
Zusatzinfo | XI, 240 p. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Allgemeines / Lexika |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Naturwissenschaften ► Biologie ► Zellbiologie | |
ISBN-10 | 1-4939-6153-5 / 1493961535 |
ISBN-13 | 978-1-4939-6153-5 / 9781493961535 |
Zustand | Neuware |
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