Disease Gene Identification

Technological Issues and Experimental Design of Gene Association Studies.- Statistical Issues in Gene Association Studies.- Identification of Causal Sequence Variants of Disease in the Next Generation Sequencing Era.- Microarray-Based Genome-Wide Association Studies Using Pooled DNA.- Medium-Throughput SNP Genotyping Using Mass Spectrometry: Multiplex SNP Genotyping Using the iPLEX® Gold Assay.- Targeted SNP Genotyping Using the TaqMan Assay.- Bar-Coded, Multiplexed Sequencing of Targeted DNA Regions Using the Illumina Genome Analyzer.- Site-Directed Mutagenesis.- Gene Expression Profiling of Tissues and Cell Lines: A Dual Color Microarray Method.- Methods for microRNA Microarray Profiling.- Allelic Expression Profiling to Dissect Genome-Wide Association Study Signals.- Quantitative Polymerase Chain Reaction: Quantitative PCR Using the Comparative Cq Method.- Genomic Analysis by Oligonucleotide Array Comparative Genomic Hybridization Utilizing Formalin-Fixed Paraffin Embedded Tissues.- RNA Mapping Protocols: Northern-Blot and Amplification of cDNA Ends.- High Content RNA Interference Assay: Analysis of Tau Hyperphosphorylation as a Generic Paradigm.- Integrative Systems Biology Approaches to Identify and Prioritize Disease and Drug Candidate Genes.- Identification of a Common Variant Affecting Human Episodic Memory Performance Using a Pooled Genome-Wide Association Approach: A Case Study of Disease Gene Identification.- RNAi-Based Functional Pharmacogenomics.- Genetic Predisposition to β-Thalassemia and Sickle Cell Anemia in Turkey: A Molecular Diagnostic Approach.