Surface Plasmon Resonance -

Surface Plasmon Resonance

Methods and Protocols
Buch | Softcover
286 Seiten
2016 | Softcover reprint of the original 1st ed. 2010
Humana Press Inc. (Verlag)
978-1-4939-6125-2 (ISBN)
139,09 inkl. MwSt
In this book, experts in the field present a wide variety of applications involving commercially available SPR instruments. The book fills a need for well-described, hands-on SPR experimental protocols, and it highlights the backgrounds of vital issues.

Surface Plasmon Resonance: A General Introduction.- The Role of Mass Transport Limitation and Surface Heterogeneity in the Biophysical Characterization of Macromolecular Binding Processes by SPR Biosensing.- Amine Coupling Through EDC/NHS: A Practical Approach.- High-Affinity Immobilization of Proteins Using Biotin- and GST-Based Coupling Strategies.- A Capture Coupling Method for the Covalent Immobilization of Hexahistidine Tagged Proteins for Surface Plasmon Resonance.- Affinity Constants for Small Molecules from SPR Competition Experiments.- Surface Plasmon Resonance Signal Enhancement for Immunoassay of Small Molecules.- High-Throughput Kinase Assay Based on Surface Plasmon Resonance.- SPR Biosensor as a Tool for Screening Prion Protein Binders as Potential Antiprion Leads.- Carbohydrate–Lectin Interactions Assayed by SPR.- DNA Sensors Based on Mixed Self-Assembled DNA/Alkanethiol Films.- Preparation of Lipid Membrane Surfaces for Molecular Interaction Studies by Surface Plasmon Resonance Biosensors.- Capture of Intact Liposomes on Biacore Sensor Chips for Protein–Membrane Interaction Studies.- Surface Plasmon Resonance Spectroscopy for Studying the Membrane Binding of Antimicrobial Peptides.- Surface Plasmon Resonance Spectroscopy in Determination of the Interactions Between Amyloid ? Proteins (A?) and Lipid Membranes.- Incorporation of a Transmembrane Protein into a Supported 3D-Matrix of Liposomes for SPR Studies.- Application of Surface Plasmon Resonance Spectroscopy to Study G-Protein Coupled Receptor Signalling.- Integration of SPR Biosensors with Mass Spectrometry (SPR-MS).- SPR/MS: Recovery from Sensorchips for Protein Identification by MALDI-TOF Mass Spectrometry.

Erscheinungsdatum
Reihe/Serie Methods in Molecular Biology ; 627
Zusatzinfo X, 286 p.
Verlagsort Totowa, NJ
Sprache englisch
Maße 178 x 254 mm
Themenwelt Naturwissenschaften Biologie Biochemie
Naturwissenschaften Biologie Zellbiologie
ISBN-10 1-4939-6125-X / 149396125X
ISBN-13 978-1-4939-6125-2 / 9781493961252
Zustand Neuware
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